壮族药旱田草质量标准  

Quality Standards of Zhuang Medicine Lindernia ruellioides

作　　者：黎理[1] 唐玉荣[1] 颜萍花 蔡毅[1] 

机构地区：[1]广西中医药大学,南宁530001

出　　处：《中国实验方剂学杂志》2016年第21期36-40,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：<广西壮药质量标准(第三卷)>质量评价与标准研究项目(MZY 2013022);广西自然科学基金项目(2011GXNSFF018006);广西"2011协同创新中心"-壮瑶药协同创新中心项目(桂教科研[2013]20号);广西壮瑶药重点实验室项目(桂科基字[2014]32号);广西重点学科(壮药学)项目(桂教科研[2013]16号);"八桂学者"工程专项

摘　　要：目的:建立旱田草药材的质量控制标准,为其药用植物资源的开发利用提供科学依据。方法:对旱田草药材进行性状及显微鉴别研究;以毛蕊花糖苷为对照品,采用薄层色谱法对旱田草进行定性鉴别;按2015年版《中国药典》附录方法对各产地药材进行水分、总灰分、酸不溶性灰分、浸出物测定;采用高效液相色谱法测定旱田草中毛蕊花糖苷的含量。结果:对旱田草药材的性状、显微特征进行了描述,且薄层色谱图中各斑点分离度好、清晰,鉴别特征稳定可靠;拟订旱田草药材中水分不得过13.00%,总灰分不得过21.00%,酸不溶性灰分不得过10.00%,醇溶性浸出物不得少于15.00%;毛蕊花糖苷的质量分数不得少于0.20%。结论:所建立的方法操作简便、准确可靠,重复性好,制订的标准限度合理,可用于旱田草药材的质量控制。Objective： To provide the scientific basis for the utilization and development of Lindernia ruellioides by formulating the quality control standards for it. Method： The characteristics and microscopic identification of L. ruellioides were studied. With verbascoside as the reference substance,TLC was applied in the qualitative identification of it. Moisture, total ash, acid insoluble ash and extractives in L. ruellioides was determined based on the approaches in the appendix of Chinese Pharmacopoeia published in 2015,and the content of verbascoside was analyzed by HPLC. Result： The characteristics and microscopic identification of L. ruellioides were described,with stable and reliable identification characteristics. The spots in TLC were clear,with a good separation. The limits for moisture,total ash and acid insoluble ash in L. ruellioides shall be less than 13. 00%,21. 00% and 10. 00%,respectively,and the limits for the extractives and the content of verbascoside shall be more than 15. 00% and 0. 20%. Conclusion： The method established is simple,accurate and reliable,and the standard limit is reasonable,whic can be used to control the quality of L. ruellioides.

关 键 词：旱田草 显微鉴别 毛蕊花糖苷 质量标准 

分 类 号：R284.1[医药卫生—中药学]