疏肝健脾法肠激安方对IBS-D大鼠免疫功能的影响  

Effect of Shugan Jianpi Method(Changji'an Fomulation) on IBS-D Immune Function

作　　者：柴玉娜[1] 黄育生[1] 唐洪梅[2] 王婷[1] 刘媛[1] 钟如帆 

机构地区：[1]广州中医药大学,广州510405 [2]广州中医药大学第一附属医院,广州510405

出　　处：《中国实验方剂学杂志》2016年第21期93-98,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81073160);广东省自然科学基金项目(S2012010010539);广州中医药大学第一临床医学院优秀博士论文培育项目(YB201402)

摘　　要：目的:考察疏肝健脾法肠激安方对腹泻型肠易激综合征(IBS-D)大鼠免疫系统的影响,探讨其调控IBS-D脑-肠轴异常的免疫学机制。方法:取正常SD大鼠采用母子分离、乙酸刺激结合束缚应激的方法制造IBS-D模型,造模成功后将大鼠随机分为模型组,匹维溴铵组(0.018 g·kg^(-1)),疏肝健脾中药肠激安高、中、低剂量组(33.48,16.74,8.37 g·kg^(-1)),每组6只,连续ig 14 d,另取同批次正常大鼠6只作为正常组,同法给予生理盐水。给药结束后观察各组大鼠的日进食量、糖水偏好情况、自主活动3种行为学特征,酶联免疫吸附测定法(ELISA)检测血清中炎症因子白细胞介素-8(IL-8)和IL^(-1)0的含量,实时荧光定量聚合酶链式反应(Real-time PCR)法检测结肠中肿瘤坏死因子-α(TNF-α)及IL^(-1)βmRNA的表达,并测定胸腺系数和脾系数的变化。结果:与正常组比较,IBS-D模型组大鼠的日均进食量、糖水偏好率及自主活动均减少,血清IL-8含量及结肠中TNF-α,IL^(-1)βmRNA表达量明显升高,血清IL^(-1)0含量明显降低,胸腺系数和脾系数明显增高(P<0.05);与模型组比较,各给药组日均进食量、糖水偏好率及自主活动回升,结肠中TNF-αmRNA水平降低,血清IL^(-1)0含量升高(P<0.05),匹维溴铵组和肠激安高、中剂量组IL-8含量和结肠中IL^(-1)βmRNA水平显著降低(P<0.05),而低剂量无统计学差异,各组胸腺系数显著降低(P<0.05),脾系数呈降低趋势,但无统计学差异。结论:疏肝健脾法能够改善IBS-D大鼠的肝郁脾虚状态,并能够调节炎症因子的表达和一定程度地改善免疫器官功能,这可能是其调控IBS-D脑-肠轴功能异常、治疗IBS-D的作用途径之一。Objective： To examine the effects and immunological mechanism of Shugan Jianpi method on diarrehae-irritable bowel syndrome（ IBS-D） brain-gut axis dysfunction. Method： SD rats were treated with mother-child separation,acetic acid stimulation combined with restraint stress method to establish the IBS-D model.Themodel rats were randomly divided into five groups： model control group,pinaverium bromide group（ 0. 018 g·kg^（-1））,Changji＇an high,medium and low-dose groups（ 33. 48,16. 74,8. 37 g·kg^（-1））,with six in each group.They were continuously administered by gavage for two weeks. At the same time,six normal rats were given normal saline as a normal control group. After administration,average daily food intake,percentage of sugar preferences and automatic activity were observed. ELISA assay was used to detect content of interleukin-8（ IL-8） and IL^（-1）0 in serum,real-time fluorescence quantitative PCR was used to detect tumor necrosis factor-α（ TNF-α） and IL^（-1）βmRNA expressions in colon,and determinate changes in thymus and spleen coefficients. Result： Compared with normal control group,average daily food intake,percentage of sugar preferences and automatic activity of rats in IBS-D model group were reduced,IL-8,TNF-α,IL^（-1）β expressions were increased,IL^（-1）0 reduced,thymus and spleen coefficient significantly increased（ P〈0. 05）; and compared with model group,in each treatment group,average daily food intake,percentage of sugar preferences and automatic activity significantly rebounded,TNF-αlevel was reduced in colon, and IL^（-1）0 increased（ P〈0. 05）; IL-8 and IL^（-1）β expressions of pinaverium ammonium group and Changji＇an high and median-dose groups were significantly lower（ P〈0. 05）,while lowdose group showed no significant statistical difference; In each group,thymus coefficient was significantly lower（ P〈0. 05）,and spleen coefficient was decreased,but with no statistical significance. Conclusion： Shugan Jia

关 键 词：腹泻型肠易激综合征 疏肝健脾法 肠激安方 免疫功能 脑-肠轴异常 

分 类 号：R285.5[医药卫生—中药学]