地菍口服液治疗原发免疫性血小板减少症的药效学研究  

Melastoma Dodecandrum Lour Oral Liquid on Main Pharmacodynamics in Primary Immune Thrombocytopenia

作　　者：曾荣香[1] 李宏良[1] 陈丽[1] 雷凯君[1] 侯思捷[1] 黄泳立[1] 

机构地区：[1]佛山市中医院,广东佛山528000

出　　处：《中华中医药学刊》2016年第11期2751-2753,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：佛山市科技攻关项目(201208074)

摘　　要：目的:观察地菍口服液对原发免疫性血小板减少症(primary immune thrombocytopenia,ITP)模型小鼠血小板计数、出凝血时间、骨髓巨核细胞数量、血清白细胞介素-4(IL-4)及干扰素-γ(IFN-γ)的影响。方法:取60只BALB/C小鼠随机分为6组:地菍口服液高、中、低剂量组、醋酸泼尼松组、模型组、正常对照组。以隔日注射豚鼠抗小鼠血小板血清(APS)进行造模。造模第8天开始给药,每日1次,连续14 d后处死小鼠,检测外周血小板计数、出凝血时间、骨髓巨核细胞数量、IL-4及IFN-γ水平。结果:1地菍口服液高、中、低剂量组血小板数量明显高于模型组(P<0.05),出血时间及巨核细胞计数明显低于模型组(P<0.05);地菍口服液高剂量组凝血时间低于模型组(P<0.05),但中、低剂量与模型组间比较无显著变化(P>0.05);地菍口服液高、中剂量组血小板计数高于低剂量组(P<0.05)。2模型组IL-4、IFN-γ及IFN-γ/IL-4比值水平均高于正常对照组(P<0.05),以IFN-γ及IFN-γ/IL-4值升高为主(P<0.05);地菍口服液高剂量组IL-4、IFN-γ水平及IFN-γ/IL-4值低于模型组(P<0.05),而中、低剂量组仅IFN-γ水平及IFN-γ/IL-4值低于模型组(P<0.05);地菍口服液高、中、低剂量组IL-4、IFN-γ及IFN-γ/IL-4值无明显差异(P>0.05)。结论:地菍口服液对于提升ITP小鼠血小板计数及改善出血症状有较好疗效,增加剂量有助于提高疗效。通过降低ITP小鼠INF-γ水平及IFN-γ/IL-4比值,使Th1/Th2细胞恢复平衡,可能是其起效的机制之一。Objective：To observe the effect of Melastoma dodecandrum Lour Oral Liquid on peripheral blood platelets count, bleeding time, clotting time, megacaryocyte in marrow,interleukin -4 and interferon -γ in primary immune throm- bocytopenia（ITP） mice model. Methods： Sixty BALB/C mice were randomly divided into Melastoma dodecandrum Lout Oral Liquid high, middle and low doses groups, prednisone acetate group, model group and normal control group. ITP mice models were made by intraperitoneally injecting guinea pig- anti mice platelet serum（APS） every other day. Eight days after injecting the APS, the mice were fed with medicine once a day. The peripheral blood platelets count, bleeding time, clotting time, megacaryocyte in marrow,interleukin -4 and interferon -γ were detected after 14 days. Results： ①The peripheral blood platelets count in Melastoma dodecandrum Lout Oral Liquid high, middle and low doses groups were significantly higher than that in model group （P 〈 0. 05）. The bleeding time, clotting time, megacaryocyte in Melastoma dodecandrum Lour Oral Liquid high, middle and low doses groups were significantly lower than those in model group （P 〈 0. 05）. The bleeding time in Melastoma dodecandrum Lour Oral Liquid high dose group was lower than that in model group （P 〈 0.05 ）. The peripheral blood platelets count in Melastoma dodecandrum Lout Oral Liquid high and middle do- ses groups were higher than those in low dose group（P 〈0. 05）. ②The levels of IL-4,mainly IFN -γ and IFN-γ/IL -4 in model group were higher than those in normal control group（P 〈0. 05）. The levels of IL -4 ,IFN - γ/and IFN - γ/IL -4 in Melastoma dodecandrum Lour Oral Liquid high dose group were lower than those in model group （P 〈 0. 05 ）. But in Melastoma dodecandrum Lour Oral Liquid middle and low dose group, only IFN - γ/and IFN -γ/IL - 4 were lower than those in model group （P 〈 0. 05 ）. The levels of IL -4, IFN -γ and IFN - γ/1L -4 among three doses groups had no significant

关 键 词：地菍口服液 原发免疫性血小板减少症 药效学 

分 类 号：R285.5[医药卫生—中药学]