杂交三倍体(4n♀×2n♂)泥鳅染色体组核型多态性研究  

Resreach on karyotype polymorphism of hybrid triploid(4n♀ × 2n♂) loach Misgurnus anguillicaudatus

作　　者：江振华[1] 周贺[1] 麻天宇 沈芾 林忠乔 钱聪[1] 李雅娟[1] JANG Zhenhua ZHOU He MA Tianyu SHEN Fu LIN Zhongqiao QIAN Cong LI Yajuan(China Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian Liaoning 116023, China Fisheries Technology Extension Station of Beijing, Beijing 101105, China)

机构地区：[1]大连海洋大学辽宁省海洋生物资源恢复及生境修复重点实验室,辽宁大连116023 [2]北京市水产技术推广站,北京101105

出　　处：《东北农业大学学报》2016年第10期34-40,共7页Journal of Northeast Agricultural University

基　　金：国家自然科学基金项目(31272650)

摘　　要：以杂交三倍体泥鳅(4n♀×2n♂)为研究对象,初步分析对不同发育阶段(胚胎、六月龄及十二月龄)染色体核型。结果表明,胚胎阶段整三倍体(3n=75)染色体数目为3n=75,核型公式:14M+9SM+52T,NF=98;亚三倍体(3n>75)染色体数目为3n=73,核型公式:13 M+6 SM+54 T,NF=92;超三倍体(3n>75)染色体数目为3n=77,核型公式:18 M+7 SM+52 T,NF=102。六月龄阶段整三倍体染色体数目为3n=75,核型公式:15 M+5 SM+55 T,NF=95;亚三倍体染色体数目为3n=73,核型公式:13M+6SM+54T,NF=92;超三倍体染色体数目为3n=76,核型公式:14 M+6 SM+56 T,NF=96。十二月龄阶段整三倍体染色体数目为3n=75,核型公式:16 M+6 SM+53T,NF=97;亚三倍体的染色体数目为3n=72,核型公式:13M+6SM+53T,NF=91;超三倍体的染色体数目为3n=77,核型公式:13M+6SM+58T,NF=96。研究表明,杂交三倍体(4n♀×2n♂)泥鳅不同发育阶段染色体核型整倍体及非整倍体均表现出多态性。结论为杂交三倍体泥鳅染色体组不稳定性研究提供细胞遗传学依据。This study aimed at analysis chromosome karyotype of hybird triploid loach （tetraploid ♀ × diploid ♂ ） in different development stages （early embryo, six months old and twelve months old）. The results of early embryos loach showed that chromosomes number of triploid （3n=75） was 3n=75, karyotype formula was 14M＋9SM＋52T and NF=98; chromosomes number of hypotriploid （3n〈75） was 3n=73, karyotype formula was 13M＋6SM＋54T, and NF was 92; the chromosomes number of hypertriploid （3n〉75） was 3n= 77, karyotype formula was 18M＋7SM＋52T, and NF was 102. The results of six months old loach showed that the chromosomes number of triploid was 3n=75, karyotype formula was 15M＋5SM＋55T, and NF was 95; the chromosomes number of hypotriploid was 3n=73, karyotype formula was 13M＋6SM＋54T, and NF was 92; the chromosomes number of hypertriploid was 3n=76, karyotype formula was 14M＋6SM＋56T, and NF was 96. The results of twelve months old loach showed that the chromosomes number of tdploid was 3n=75, karyotype formula was 16M＋6SM＋53T, and NF was 97; the chromosomes number of hypotriploid was 3n=72, karyotype formula was 13M＋6SM＋53T, and NF was 91; the chromosomes number of hypertriploid was 3n=77, karyotype formula was 13M＋6SM＋58T, and NF was 96. The study showed that chromosome karyotype both of euploid and aneuploid showed polymorphism of hybird triploid loach in the different development stages. The conclusion provided a cytogenetic basis for hybrid triploid loach chromosome instability.

关 键 词：泥鳅 杂交三倍体 不同发育阶段 染色体核型 

分 类 号：S917.4[农业科学—水产科学]