捻转血矛线虫线粒体COXⅠ基因序列测定及进化分析  

Molecular identification of Haemonchus contortus

作　　者：白鹏霞[1,2] 郝宝成[3] 陈长江 项海涛[1] BAI Peng-xia HAO Bao-eheng CHEN Chang-jiang XIANG Hai-tao(College of Veterinary Medicine, Gansu Agricultural University, Lanzhou Gansu 730070 Qinghai Polytechnic College of Animal Husbandry ＆ Veterinary, Xining Qinghai 812100 Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou Gansu 730050 Animal Husbandry and Veterinary Station in Huangyuan County of Qinghai Province, Huang yuan Qinghai 812100, China)

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]青海畜牧兽医职业技术学院,青海西宁812100 [3]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050 [4]青海省湟源县畜牧兽医站,青海湟源812100

出　　处：《中兽医医药杂志》2016年第5期13-16,共4页Journal of Traditional Chinese Veterinary Medicine

基　　金：国家高新技术研究发展计划(863计划)生物兽药新产品研究和创制(2011AA10A214)

摘　　要：为了对捻转血矛线虫进行分子鉴定,并且探寻其与毛圆科内不同属线虫存在差异的分子水平关联性,对采自牦牛体内的捻转血矛线虫线粒体COXⅠ基因部分序列进行扩增与进化分析,扩增出的序列登录到GenBank,登录号：KU314701。进化分析结果显示,捻转血矛线虫H-yak线粒体COXⅠ基因与毛圆科不同属线虫的线粒体COXⅠ基因同源性在85.5%～97.4%之间,其中与KJ724363.1的同源性最高,达到97.4%;其编码的蛋白氨基酸序列与血矛属线虫的线粒体细胞色素C氧化酶亚基Ⅰ蛋白的氨基酸序列完全相同,同源性为100%,且与长刺属线虫的同源性极高,同为100%,但与古柏属和奥斯特属的线虫蛋白同源性均为98.7%。该研究在基因和蛋白两个层面间接印证了毛圆科内不同属线虫在形态和生活史方面有较多相似和不同之处。To molecularly identify Haemonchus contortus and search for the differences of the different genera of nematodes in the family of Trichostrongylidae. The mitochondrial COXⅠgene was amplified by PCR and sequenced which login in Gene Bank,the serial number is KU314701. The homology and genetic evolution analysis showed that the nucleotide homology among Haemonchus contortus H-yak＇s mitochondrial COXⅠgene and other related sequences was ranged from 85.5% to 97.4%; and the homology of mitochondrial cytochrome c oxidase subunit I amino acid with related proteins of the family of Trichostrongylidae was highly conserved from 98.7% to 100%.

关 键 词：捻转血矛线虫 线粒体 COXⅠ基因 序列测定 进化分析 

分 类 号：S855.9[农业科学—临床兽医学]