人肝细胞系LO2单纯肝脂肪变性细胞模型的建立  

Establishment of a LO2 cell model of hepatic steatosis

作　　者：李姣[1] 石挺[1] 刘磊[1] 陈志刚[1] 李文平[1] LI Jiao SHI Ting LIU Lei CHEN Zhi-gang LI Wen-ping(College of Veterinary Medicine, Hunan Agricultural University, Changsha Hunan 410128, China)

机构地区：[1]湖南农业大学动物医学院,湖南长沙410128

出　　处：《中兽医医药杂志》2016年第5期20-23,共4页Journal of Traditional Chinese Veterinary Medicine

基　　金：湖南省大学生创新性试验计划项目(SCX1504)

摘　　要：目的:探讨建立人肝细胞系LO2单纯肝脂肪变性细胞模型的方法。方法:试验分为正常对照组与试验组,用含10%胎牛血清的1640培养基培养LO2细胞,当细胞处于对数生长期时,正常对照组更换新鲜的1640培养基,试验组用含不同浓度游离脂肪酸(油酸∶棕榈酸为2∶1)的1640培养基培养,24 h后油红O染色,观察细胞内脂滴大小,并检测细胞中TG、TC含量的变化及细胞增殖情况。结果:试验组中LO2细胞内有大量的脂滴生成,且甘油三酯水平显著升高,其中,以1.2 mM FFA组脂质沉积最为明显。同时,细胞抑制率小于4%,胆固醇水平无明显升高,均符合单纯脂肪变性模型的特征。结论:采用游离脂肪酸(油酸∶棕榈酸为2∶1)可以成功诱导LO2细胞单纯脂肪变性,其中游离脂肪酸浓度以1.2 m M最为理想。Objective： To establish a cell model for investigating hepatic steatosis in non-alcoholic fatty liver disease.Methods： LO2 cells cultured in 1640 containing 10% fetal bovine serum were divided into normal group and treatment group. When cells in logarithmic phase, LO2 cells in the treatment group were exposed to a long-chain mixture of free fatty acids（FFA, oleate and palmitate） for 24 h, and cells in normal group were treated with fresh medium. Lipid droplets in the cells were observed with Oil Red O staining, total lipid content and cell proliferation was determined. Results： A large number of lipid droplets were found in the cell treated, which showed markedly increased of triglyceride level without significant changes of cholesterol content. At the same time, the cell inhibition rate was less than 4%. 1.2 m M FFA group of lipid deposition was most obvious. Conclusion： Hepatic steatosis LO2 cell model can be established by long-chain mixture of free fatty acids（oleate∶palmitate=2∶1）. 1.2 m M of free fatty acid is the most ideal.

关 键 词：脂肪肝 LO2细胞 细胞模型 游离脂肪酸 

分 类 号：S855.9[农业科学—临床兽医学]