宫康中水苏碱含量测定  

Determination of Stachydrine in Gongkang perfusion

作　　者：苗小楼[1] 尚小飞[1] 王东升[1] 潘虎[1] 王瑜[1] 李升桦 MIAO Xiao-lou SHANG Xiao-fei WANG Dong-sheng PAN Hu WANG Yu(Gansu Provincial Key Laboratory of New Animal Drug Project, Engineering ＆ Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of the Ministry of Agriculture for Veterinary Pharmaceutical Development, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou Gansu 730050, Chin)

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省中兽药工程技术研究中心甘肃省新兽药工程重点实验室,甘肃兰州730050 [2]甘肃省通渭县农牧局畜牧中心,甘肃通渭743300

出　　处：《中兽医医药杂志》2016年第5期69-71,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：兰州市科技计划(项目编号2014-2-26)

摘　　要：目的:筛选宫康中水苏碱含量测定供试品溶液的制备方法,建立宫康中水苏碱HPLC-ELSD含量检测方法。方法:采用阳离子交换树脂、五氯酚、硫氰酸铬铵3种方法制备供试品溶液,采用HPLC-ELSD测定水苏碱含量。结果:采用硫氰酸铬铵制备的供试品溶液较好,水苏碱含量在2.20-11.00μg范围内线性关系良好(R^2=0.999),平均回收率为95.96%。结论:该法简便、样品纯净,灵敏,阴性无干扰,可以有效控制该制剂质量。Objective： To filter sample solution preparation method and establish assay method for Stachydrine in Gongkang perfusion. Methods： To prepare sample solution with cation exchange resin, pentachlorophenol and Reinecke salt, HPLC-ELSD was used to determine the content of Stachydrine. Results： Stachydrine had a good linearity in the concentration range of 2.20-11.00 μg（R2=0.999） when the sample solution was prepared with Reinecke salt. The average recovery was 95.96%. Conclusion： This filtered sample preparation method is simple, and the prepared sample solution is pure. HPLC-ELSD analysis is accurate and reliable with high sensitivity.

关 键 词：子宫内膜炎 宫康 HPLC-ELSD 水苏碱 

分 类 号：S853.7[农业科学—临床兽医学]