UPLC同时测定墨旱莲药材中8种成分的含量  

Simultaneous determination of 8 main compounds in Eclipta Herba by UPLC

作　　者：侯雪峰[1,2] 孙帅[2] 汪刚[1,2] 邱辉辉[2] 丁淑敏[3] 刘莹[1,2] 刘丹[2] 封亮[2] 贾晓斌[2] 

机构地区：[1]安徽中医药大学,安徽合肥230012 [2]江苏省中医药研究院国家中医药管理局中药释药系统重点研究室,江苏南京210028 [3]常州大学制药与生命科学学院,江苏常州213164

出　　处：《中国中药杂志》2016年第21期3982-3987,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81473394;81503265;8160336);江苏省"333高层次人才培养工程"项目(BRA2015475)

摘　　要：建立一种超高效液相色谱法同时测定墨旱莲中的异槲皮苷、木犀草苷、异去甲蟛蜞菊内酯、异绿原酸A、异绿原酸C、木犀草素、蟛蜞菊内酯、芹菜素8种成分含量的方法。采用Waters Acquity UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7μm),流动相A为乙腈,B为0.1%的甲酸水,梯度洗脱条件:0~4 min,10%~13%A;4~10 min,13%~16%A;10~13 min,16%~25%A;13~17 min,25%~28%A;17~20 min,28%~40%A;20~25 min,40%~95%A。流速:0.3 m L·min^(-1);柱温35℃;检测波长350 nm。研究结果表明各指标成分之间分离度良好,在选定的浓度范围内线性关系良好(r≥0.999 0),平均加样回收率(n=6)在96.60%~103.4%,RSD为0.86%~2.4%。所建立的UPLC同时测定墨旱莲药材中8种指标成分的含量的方法回收率较好,重复性、稳定性良好,为墨旱莲的鉴别及质量评价提供了科学依据。To establish an UPLC method for the simultaneous determination of 8 compounds in Eclipta Herba,such as isoquercitrin,luteoloside,demethylwedelolactone,isochlorogenic acid A,isochlorogenic acid C,luteolin,wedelolactone and apigenin.The experiment was performed with a Waters Acquity UPLC BEH C18（ 2. 1 mm × 100 mm,1. 7 μm） column by gradient elution of 0. 1 % formic acid in water and acetonitrile： 0-4 min,10 %-13 % A; 4-10 min,13 %-16 % A; 10-13 min,16 %-25 % A;13-17 min,25 %-28 % A; 17-20 min,28 %-40 % A; 20-25 min,40 %-95 % A. The flow rate was 0. 3 m L·min^（-1）. The condition of was the colum temperature was maintained at 35 ℃ and the detected wavelength was set at 350 mm. 8 components were separated clearly by this method. Also a good linearity was obtained between the chosen concentration（ r≥0. 999 0）. The measured data showed that the recovery rate range from 96. 60 %-103. 4 %（ n = 6） and their RSD values were 0. 86 %-2. 4 %. The method has high recovery rate,good reproducibility and stability. It provides a scientific basis for the identification and quality evaluation of Eclipta Herba.

关 键 词：墨旱莲 超高液相色谱法 多指标成分 含量测定 

分 类 号：R284.1[医药卫生—中药学] O657.7[医药卫生—中医学]