苹果全基因组多聚半乳糖醛酸酶基因家族的鉴定及进化分析  

Identification and Phylogenetic Analysis of the POLYGALACTURONASE Gene Family in Apple

作　　者：陈鸿飞[1] 邵红霞[1] 樊胜[1] 马娟娟[1] 张东[1] 韩明玉[1] CHEN Hong-fei SHAO Hong-xia FAN Sheng MA Juan-juan ZHANG Dong and HAN Ming-yu(College of Horticulture, Northwest A ＆ F University, Yangling , Shaanxi 712100, China)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《园艺学报》2016年第10期1863-1877,共15页Acta Horticulturae Sinica

基　　金：国家科技支撑计划项目(2013BAD20B03);国家星火计划项目(2014GA850002);国家现代农业产业技术体系建设专项资金项目(CARS-28);国家苹果改良中心杨凌分中心;陕西省农业科技创新与攻关项目(2015NY114);陕西省果业发展协同中心和陕西果业发展项目

摘　　要：从苹果全基因组中鉴定出85个多聚半乳糖醛酸酶(PG)基因,通过聚类分析将其分成了7个组(Group A^Group G),其分子量在176~1 125 aa之间,等电点在4.68~9.58之间。鉴定出来的Md PG基因除在14号染色体没有分布外,其余都有分布。系统分析了Md PG蛋白的保守基序和Md PG基因结构,发现苹果PG蛋白除包含有广泛存在于各种PG蛋白的4个保守基序以外,还含有其他相对特异的保守基序,Md PG75具有最多的外显子数,达到18个。对85个苹果PG蛋白之间的功能联系网络进行了系统研究,分析预测了相关基因的功能和多个基因间功能的联系,并作了初步验证。In this study,a total of 85 apple polygalacturonase genes were characterized and clustered into seven groups based on the Malus × domestica whole-genome sequence. These coded for proteins containing 176–1 125 amino acids with isoelectric points ranging from 4.68–9.58. The predicted Md PG genes were distributed on all chromosomes except the 14 th. We then systematically analyzed conserved Md PG protein motifs and the structures of Md PG genes. We identified Md PG proteins containing four conserved motifs that are widely found in different PG proteins. Additionally,we found that Md PG75 was the largest gene,encompassing 18 exons. Finally,we systematically analyzed the functional connection network of Md PG proteins and predicted the functions of related Md PG genes before undertaking a preliminary validation. Overall,we have described the genome-wide identification and analysis of the apple PG gene family.

关 键 词：苹果 PG 家族分析 生物信息学 

分 类 号：S666.1[农业科学—果树学]