鸡和缓艾美耳球虫早熟虫株选育及其生物学特性研究  

Selection and Biological Characteristic of Precocious Strain of Chicken Eimeria mitis

作　　者：王娟 刘永清 高永刚 梁伟 闵长安 付改玲 张文叶 郑润宽[2] WANG Juan LIU Yong-qing GAO Yong-gang LIANG Wei MIN Chang-an FU Gai-ling ZHANG Wen-ye ZHENG Run-kuan(Inner Mongolia Shenyuan Bioengineering Co.,Ltd., Hohhot 010010,China Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)

机构地区：[1]内蒙古神元生物工程股份有限公司,内蒙古呼和浩特010010 [2]内蒙古农牧业科学院,内蒙古呼和浩特010031

出　　处：《畜牧与饲料科学》2016年第9期15-18,共4页Animal Husbandry and Feed Science

基　　金：呼和浩特市重大科技专项项目(2011-合-4)

摘　　要：按照Jeffers1975年建立的艾美耳属球虫早熟虫株选育方法,对鸡和缓艾美耳球虫巴彦淖尔株(SY-MB株)进行了单卵囊分离、扩增和早熟虫株的选育。经SPF鸡15代传代之后,分离株的潜隐期由96 h缩短至77 h,缩短了19 h。按索勋等1998年建立的方法对选育出的和缓艾美耳球虫巴彦淖尔早熟株(SY-mb77株)进行10代非选择性传代,证明该早熟虫株的潜隐期、卵囊产量、免疫原性和致病性均具备早熟虫株特性,并能有效抵抗强毒虫株的攻击。The single oocyst separation, propagation and precocious strain selection were conducted in a strain of chicken Eimeria mitis designated as SY-MB which was isolated from Bayannur region of Inner Monlolia according to the methods established by Jeffers in 1975. After 15 successive generations inoculation, the latent period of the isolated strain was 19 hours shorted （from 96 hours to 77 hours）. The selected precocious strain designated as SY-mb77 was unselectively sub-cultured for 10 generations by the methods reported by Sunxun and others in 1998. The latent period, oocyst production, immunogenicity and pathogenicity of the selected strain possessed the characteristic of a typical precocious strain. The precocious strain can effectively protect the inoculated chickens from invasion of high virulent strain.

关 键 词：鸡和缓艾美耳球虫 早熟虫株 选育 生物学特性 

分 类 号：S852.72[农业科学—基础兽医学] S852.43[农业科学—兽医学]