HPLC-MS法测定罗汉果中10种氨基甲酸酯类农药  

Determination of 10 Kinds of Carbamate Pesticides in Siraitia grosvenorii by HPLC-MS

作　　者：付明磊[1] 陈旭[1] 周先丽[1] 黄晓[1] 程柳[2] 罗琴[1] 白红妍[1] 谭微[1] 李远远[1] 梁成钦[1] FU Ming-lei CHEN Xu ZHOU Xian-li HUANG Xiao CHENG Liu LUO Qin BAI Hong-yan TAN Wei LI Yuan-yuan LIANG Cheng-qin(Guangxi Key Laboratory of Pharmacognosy in University, Guilin Medical University, Guilin 541004, Guangxi, China China Nonferrous Metals （Guilin） Geology and Mining Co., Ltd., Guilin 541004, Guangxi, China)

机构地区：[1]桂林医学院广西高校生药学重点实验室,广西桂林541004 [2]中国有色桂林矿产地质研究院有限公司,广西桂林541004

出　　处：《食品研究与开发》2016年第16期133-136,共4页Food Research and Development

基　　金：广西科学研究与技术开发计划项目(桂科能14123006-21和桂科能1598025-18)

摘　　要：建立HPLC-MS/MS分析方法,同时测定罗汉果中10种氨基甲酸酯农药残留量。样品经有机溶剂超声提取,提取液浓缩后经固相萃取小柱净化,采用梯度洗脱分离,色谱系统条件为VP-ODS色谱柱(2.0 mm×150 mm,5μm),柱温40℃,流速0.2 m L/min,进样量10μL,以多反应检测模式监测。考察了3个水平添加下(250、500、1000μg/L),10种农药的平均回收率为84.5%~111.7%,相对标准偏差为1.8%~7.3%,检测限在0.1μg/kg^2.0μg/kg。该方法准确、快速、抗干扰能力强,可作为罗汉果中氨基甲酸酯类农药残留的测定。HPLC-MS was employed for determination of carbamate pesticides in Siraitia grosvenorii. The sam-ples were cleaned with solid-phase extraction column after ultrasonic extraction. The extracts were separated by gradient elution. The condition were VP-ODS column （2.0 mm×150 mm,5μm）,temperature 40℃,speed 0.2 mL/min, inject 10μL. Pesticides were detected with multiple reaction monitoring mode. In 3 different degrees（250,500, 1 000μg/L）,the average recoveries were 84.5%-111.7%,the relative standarddeviation were 1.8%-7.3%, the detection limits were 0.1μg/kg-2.0μg/kg. The method is accurate,rapid,anti-interference and suitable for determination of these pesticides in Siraitia grosvenorii.

关 键 词：罗汉果 液相色谱串联质谱 氨基甲酸酯类农药 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]