粳稻品种Oochikara稻瘟病抗性基因挖掘  

Rice blast-resistant gene mining in japonica rice cultivar Oochikara

作　　者：徐小金[1,2] 董波[3] 陶跃之[2] 王华[2] XU Xiao-jin DONG Bo TAO Yue-zhi WANG Hua(College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China Institute of Virology and Biotechnology l Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021 [3]浙江省农业科学院病毒学与生物技术研究所,浙江杭州310021

出　　处：《江苏农业学报》2016年第5期961-967,共7页Jiangsu Journal of Agricultural Sciences

基　　金：转基因生物新品种培育重大专项(2012ZX08009001)

摘　　要：对水稻品种Oochikara(Oryza sativa L.ssp.japonica)进行稻瘟病抗性分析,发现Oochikara对来自不同地区的30个稻瘟菌菌株具有高效的广谱抗性。利用等位基因挖掘技术,从Oochikara鉴定出4个已知稻瘟病抗性基因(R gene):Pish、Pia、Pikm和Pita。此外,利用稻瘟菌菌株08-7-3对Oochikara和感病品种E103杂交后代进行稻瘟菌喷雾接种和遗传分析,发现Oochikara还存在其他新的抗性基因。以上研究结果初步揭示了Oochikara对稻瘟病的抗性来源,有助于利用Oochikara作为抗性中间材料开展稻瘟病抗性育种工作。A rice japonica cultivar Oochikara confers broad spectrum resistance to thirty Magnaporthe grisea strains collected from different regions. Four known rice blast resistance genes （ R gene） , Pish,Pia,Pikm and Pita were identified from Oochikara by allele mining approach. Moreover, genetic analysis and inoculation with the M. grisea strain 08-7-3 by spraying were performed in the progenies derived from a cross of Oochikara and a blast susceptible cultivar E103 showed that there were some other novel R gene （ s ） in Oochikara. The findings revealed the source of rice blast resistance in Oochikara, which will facilitate the development of elite cultivars against rice blast using Oochikara as an intermediate ma-terial for breeding.

关 键 词：稻瘟菌 抗性基因 等位挖掘 喷雾接种 

分 类 号：S511.220.353[农业科学—作物学]