抗条锈病小麦-中间偃麦草-华山新麦草杂种后代的分子细胞遗传学鉴定  

Molecular Cytogenetic Characterization of the Derivatives from Wheat-Thinopyrum intermedium-Psathyrostachys huashanica Hybrids with Resistance to Stripe Rust

作　　者：王艳丽 王伟 陈天青 隋建枢 何庆才[2] 

机构地区：[1]贵州省旱粮研究所,贵阳550006 [2]贵州省农业科学院,贵阳550006

出　　处：《种子》2016年第10期6-9,共4页Seed

基　　金：国家小麦产业技术体系经费资助(编号:CARS-2-43)

摘　　要：小麦近缘物种中蕴藏着许多优良基因,创制三属杂种是小麦遗传改良的重要途径。对4个F6代株系采用农艺性状调查、PMC染色体配对观察、GISH和SDS-PAGE等方法分析。结果表明,4个株系的染色体数目均为42条,K-13-649-3、K-13-663-2在细胞遗传学上相对稳定;且均确证为小麦-中间偃麦草易位系;K-13-649-3、K-13-728-4与亲本中3的特异条带一致,K-13-656-3、K-13-663-2只有3条条带,缺失了亲本中3的1条特异条带,4个株系表现为高抗条锈病。The relatives of wheat possess a large number of useful genes,Creating trigeneric hybrids is an important way for inherited improvement.In this study,Four F6 lines derived from the cross of PHW-SA×Zhong 3were identified using agronomic traits investigation,cytogenetics,GISH and SDSPAGE.The results showed that all lines had 42 chromosomes,Lines K-13-649-3and K-13-663-2were stable on the cytogenetics.These 4lines were wheat-Th.intermediumtranslocation lines.K-13-649-3and K-13-728-4expressed specific glutenin bands consistent with their parent Zhong 3.K-13-656-3and K-13-663-2only expressed three bands,one specific glutenin band was disappeared in these two lines.All lines showed high resistance to stripe rust.

关 键 词：中间偃麦草 华山新麦草 基因组原位杂交 易位系 

分 类 号：S512.1[农业科学—作物学]