双层细菌纤维素基组织工程尿道支架的设计与初步构建  

Design and Fabric ation of Abacterial Cellulose Bilayer Scaffold for Urethral Tissue Engineering

作　　者：李喆[1,2] 吕向国[3] 王宝秀[1] 冯超[3] 陈仕艳[1] 颜志勇[2] 王华平[1] 

机构地区：[1]东华大学材料学与工程学院纤维材料改性国家重点实验室,上海市201620 [2]嘉兴学院材料与纺织工程学院,浙江省嘉兴市314001 [3]上海交通大学附属第六人民医院泌尿外科,上海市200233

出　　处：《组织工程与重建外科杂志》2016年第5期276-280,共5页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：博士创新基金(CUSF-DH-D-2015029);国家自然科学基金(51573024)

摘　　要：目的设计并构筑具有模拟尿道组织微环境的三维微／纳支架材料。方法通过对尿道脱细胞基质多尺度结构分析，设计仿尿道组织的双层细菌纤维素（Baterial cellulose，BC）基微／纳组织工程尿道支架。采用模板合成法。以明胶多孔支架为模板．在其上静态培养制备兼具微米多孔与纳米纤维的双层BC基微／纳支架材料。结果双层BC基微／纳支架材料的致密层为细菌纤维素膜，疏松层为相互连通的多孔结构，平均孔径为（167±56）μm，同时在其微米级孔壁表面修饰着模拟天然细胞外基质（Extra cellular matrix，ECM）结构的纳米纤维网络。纳米纤维直径为（20～40）nm。结论采用模板合成法．成功构建出了兼具宏观双层结构、微米级多孔和纳米级纤维的双层BC微／纳组织工程尿道支架。Objective To design and fabrication of the 3D scaffolds that exhibit biomimetic muhi-scale structures composed of microporous structure with nanofibers for urethral tissue. Methods By analyzing the multi-scale structures of urethral acellular matrix, a bilayer scaffold composed of microporous section and nanofibrous dense section to biomimetic urethral tissue was designed. Template biosynthesis was used to fabricate 3D microporous nanofibrous gelatin/BC bilayer scaffolds （BC-Gel/BC） by stationary cultivation using microporous gelatin scaffold as a template. Results The BC-Gel/BC bilayer scaffold composed of dense section of BC nanofibrils network and microporous section of Gel/BC. The microporous section with highly interconnected micropore （167±56）μm and surface decorated on the micropore wall by BC nanofibers （20-40） nm were fabricated, which were remarkably similar structure to the native extracellular matrix （ECM）. Conclusion Using the template biosynthesis method, the BC-Gel/BC bilayer scaffold for urethral tissue engineering can be successfully constructed, which has the bilayer structure, micro porous and nanofibrous network to biomimetic urethral tissue.

关 键 词：尿道组织工程 脱细胞基质 细菌纤维素 多尺度结构 

分 类 号：Q813.12[生物学—生物工程]