截留相对分子质量≤1000半夏白术天麻汤4种提取液对原发性高血压大鼠血清中单胺类递质的影响  

Effect of four extracting solutions in Banxia Baizhu Tianma Decoction with relative molecular mass ≤ 1 000 on monoamines neurotransmitters in serum of rats with primary hypertension

作　　者：王淑玲[1] 徐男 孙云廷[3] 孙剑锋[1] 周晓晓[1] 谢恬[1] WANG Shu-ling XU Nan SUN Yun-ting SUN Jian-feng ZHOU Xiao-xiaol XIE Tian(Medicine College of Hangzhou Normal University, Hangzhou 310012, China Key disciplines on Analysis of Traditional Chinese Medicine, Shandong Academy of Chinese Medicine, Jinan 250014, China Hangzhou Hospital of Traditional Chinese Medicine （Guangxing Hospital Affiliated to Zhejiang Chinese Medical University）, Hangzhou 310012, China)

机构地区：[1]杭州师范大学医学院,浙江杭州310012 [2]山东中医药研究院国家中医药管理局中药分析重点学科,山东济南250014 [3]杭州市中医院(浙江中医药大学附属广兴医院),浙江杭州310012

出　　处：《药物评价研究》2016年第5期775-780,共6页Drug Evaluation Research

基　　金：浙江省自然科学基金(No.LY16H270018);国家科技部国际合作项目(2007DFA31180)

摘　　要：目的比较半夏白术天麻汤不同提取方法对大鼠血清中单胺类递质水平的影响。方法原发性痰湿壅盛型高血压大鼠,随机分为模型组、半仿生-酶法提取液(SBEE)组、水提液(SBE)组、醇提液(WE)组、半仿生提取液(AE)组,4种提取液均过中空纤维膜截留相对分子质量≤1 000。模型组饮用去离子水,不限量;SBEE组、SBE组、WE组、AE组分别ig相应提取液,每天1 m L(2.83 g/m L);正常血压大鼠8只作为对照组。喂养4周后,采用高效液相色谱-电化学(HPLC-ECD)法测定血清中单胺递质去甲肾上腺素(NE)、5-羟色胺(5-HT)水平。结果 4种提取液均可降低高血压大鼠NE和5-HT水平,与模型组比较差异显著(P<0.01),对NE影响顺序是:SBEE>SBE>AE>WE,对5-HT影响顺序是:SBEE>SBE>WE>AE。结论半夏白术天麻汤4种方法提取均可降低高血压大鼠单胺递质水平,且加入酶解过程的SBEE提取方法效果最好。Objective To investigate the effect of four extracting solutions in Banxia Baizhu Tianma Decoction（BBTD） with relative molecular mass ≤ 1 000 on levels of monoamine neurotransmitters in rat plasma. Method Primary phlegm dampness type hypertensive rats were randomly divided into model group, SBEE group, SBE group, WE group, and AE group, respectively, by ig administration of corresponding semi-bionic enzymetic extract, water extract, alcohol extract, and semi-bionic extraction liquid, 1 m L（2.83 g/m L） daily. Rats with normal blood pressure were used as control group. After four weeks, the norepinephrine（NE） and 5-serotonin（5-HT） content of serum were determined by HPLC method. Results Compared with model group, totally four extracting solutions of BBTD could reduce NE and 5-HT levels significantly in hypertension rats（P 0.01）, order of the effect was SBEE SBE AE WE and SBEE SBE WE AE, respectively. Conclusion Totally four extracting solutions of BBD can reduce monoamine transmitters in hypertension rats and SBEE extraction is better.

关 键 词：半夏白术天麻汤 单胺类递质 去甲肾上腺素 5-羟色胺 高效液相色谱-电化学法 

分 类 号：R917[医药卫生—药物分析学]