运动提高健康老年男性内皮祖细胞内皮损伤修复能力,改善血管内皮功能  

Physical exercise enhances endothelial repair capacity of endothelial progenitor cells and improves endothelial function in elderly healthy men

作　　者：夏文豪[1] 张小勤[1] 苏晨[1] 梁建文[1] 李琰[1] 张小宇[1] 余冰波 陶军[1] 

机构地区：[1]中山大学附属第一医院高血压血管病科,广东广州510080

出　　处：《中华高血压杂志》2016年第9期869-873,共5页Chinese Journal of Hypertension

基　　金：国家自然科学基金(81200249;31370941;31530023);广东省科技计划项目(2016A020215056);中山大学附属第一医院优秀青年人才计划(2012年);中山大学青年教师培育项目(14ykpy15)

摘　　要：目的探讨运动对健康老年男性内皮祖细胞(EPC)内皮损伤修复能力及内皮功能的影响。方法入选52名无心血管病危险因素的临床证据、未服用任何药物的健康老年男性,随机分为对照组[24人,年龄(67.8±3.8)岁]和试验组[28人,年龄(68.9±4.1)岁]。试验组参与12周改良布鲁斯平板运动试验(每周3次,30min/次),对照组不参与运动试验。采用密度梯度离心法分离提取EPC,采用改良Boyden小室法检测运动前后EPC体外迁移能力;观察运动前后荧光标记的EPC黏附到脐静脉内皮细胞单层数量,分析其体外黏附能力;通过裸鼠颈动脉内膜损伤模型,探讨运动前后EPC在体内皮损伤修复能力,同时检测运动前后血流介导的血管舒张功能(FMD)。结果12周的运动锻炼后,试验组老年受试者FMD升高[运动后(8.6±2.2)%比运动前(6.7±1.4)%;P<0.01],其EPC体外的迁移、黏附能力增强,在体的内皮损伤血管再内皮化面积增大[运动后(53.4±9.1)%比运动前(35.9±4.9)%;P<0.01]。Pearson相关分析结果显示,改善的FMD与EPC内皮损伤修复能力提高呈正相关(r=0.88,P<0.01),对照组以上指标差异无统计学意义。结论体育运动明显改善健康老年男性血管内皮功能,其机制与内源性EPC内皮损伤修复能力增强相关。Objective To investigate the effect of physical exercise on the endothelial repair capacity of endothelial progenitor cells （EPC） and endothelial function in elderly healthy men. Methods Fifty-two elderly healthy male subjects without clinical evidences of cardiovascular risk factors and without concomitant medications were enrolled into the study. All subjects were randomly divided into control group [（67.8±3.8} years old, n=24] or treatment group [（68.9 ± 4.1} years old, n = 28]. Subjects of treatment group underwent a 12 weeks of modified Bruce treadmill exercise test （30 min/d for 3 days per week）. EPC were isolated from peripheral blood of all subjects by density gradient centrifugation method and cultured. The migration of EPC was detected by modified Boyden cham- ber assay and the adhesion of EPC was evaluated by calculating the number of fluorescently labeled EPC adhered to human umbilical vein endothelial cells before and after exercise test. Nude mice carotid arterial intimal injury model was used to observe reendothelialization capacity in vivo of EPC. And flow-mediated dilatation （FMD） of subjects were observed before and after 12 weeks Bruce treadmill exercise test. Results FMD level was higher [after exer- cise： （ 8. 6 ± 2.2 ）% vs before exercise ： （ 6.7 ±1.4 ） % ; P〈 0.01 ], the migration and adhesion function were im- proved, and the reendothelialization area of EPC was bigger [after exercise： （53.4 ± 9.1 ）% vs before exercise （35.9±4.9） % ;P〈0.01] in healthy elderly subjects after 12 weeks physical exercise than before exercise. Pear- son correlation analysis showed that improved FMD was positively correlated with the increased reendothelialization area of EPC （r=0.88, P〈0.01）. However,the FMD, in vitro and in vivo functional activities of EPC from con- trol group had no significant statistical difference. Conclusion Physical exercise improves obviously endothelial function in elderly healthy men, which may result from enhancement of endotheli

关 键 词：运动 内皮祖细胞 内皮损伤 内皮修复 血管内皮功能 

分 类 号：R54[医药卫生—心血管疾病]