单因素和正交试验法优选大叶藓(Rhodobryum roseum)傅里叶红外光谱处理条件  

Optimization of FITR spectrometry conditions of Rhodobryum roseum by single-factor test and orthogonal experiment

作　　者：曹珍[1,2] 王振杰[1] 赵建成[2] CAO Zhen WANG Zhen-jie ZHAO Jian-cheng(Hebei College of Industry and Technology, Shijiazhuang Hebei 050091 ,China College of Life Science, Hebei Normal University, Shijiazhuang Hebei 050016 ,China)

机构地区：[1]河北工业职业技术学院,河北石家庄050091 [2]河北师范大学,河北石家庄050016

出　　处：《河北省科学院学报》2016年第3期67-74,共8页Journal of The Hebei Academy of Sciences

基　　金：河北省高等学校科学技术研究项目(Z2014001)

摘　　要：目的:优选大叶藓(Rhodobryum roseum(Hedw.)Limpr.)红外光谱处理条件。方法:运用单因素考察和正交试验相结合的形式,采用L16(45)正交设计法以样品的粉碎粒度(A)、样品与KBr混合比例(B)、样品压片质量(C)、压片压力(D)、压片时间(E)5个因素,每个因素选取4个水平进行试验。结果:样品的粉碎粒度和样品与KBr混合比例两个因素对红外光谱共有峰的吸收率影响最显著。结论:大叶藓红外光谱最佳处理条件为:样品粉碎后过200目(75μm)筛,样品与KBr混合比例为4∶100、样品压片质量30mg、压片压力10MPa、压片时间30S。Objective： To optimize the best experiment conditions of Rhodobryum roseum by Fourier transform infrared（FTIR） spectrometry. Methods： In the method of single-factor and orthogonal investigate test, L16 （4s） the effect of of the sample,tabletti tion of the sample and orthogonal experiment was designed with five factors at four levels to particle size,the mixing proportion of the sample and KBr,the weight pressure and time. Results：The particle size and the mixing propor- Br had significant effect on the absorbance of the common peaks. Conclusion：The optimal FTIR spectrometry conditions for the R. roseum were that the crude sample was powdered and passed 200 mesh sieve（75μm）,the mixing proportion of the sample and KBr was 4 ： 100,the tabletting weight was 30mg,the tabletting pressure was 10 MPa,the tabletting time was 30S.

关 键 词：正交试验 单因素试验 大叶藓 FTIR检测 

分 类 号：TQ461[化学工程—制药化工]