四磺基铝酞菁与爱尔新蓝的缔合作用在核酸定量中的应用  

Determination of Nucleic Acids Based on Shifting the Association Equilibrium between Tetrasulphonated Aluminum Phthalocyanine and Alcian Blue

作　　者：魏玲[1] 杨黄浩[1] 陈小兰[1] 曲会英[1] 许金钩[1] 

机构地区：[1]厦门大学化学系,现代分析科学教育部重点实验室,厦门361005

出　　处：《分析化学》2002年第8期946-949,共4页Chinese Journal of Analytical Chemistry

摘　　要：阴离子荧光染料四磺基铝酞菁与阳离子荧光染料爱尔新蓝的缔合作用 ,使四磺基铝酞菁发生荧光猝灭 ,而当核酸存在时 ,染料缔合平衡受到影响而导致四磺基铝酞菁的荧光恢复。根据这一原理 ,建立了核酸定量测定的新方法。方法具有很高的灵敏度和较好的选择性 ,其线性范围为 0～ 2 0 0 μg/L ;检测限分别为 1.8μg/L(SMDNA)、2 .0 μg/L(CTDNA)、5 .4μg/L(酵母RNA)。将方法用于实际样品金黄色葡萄球菌中DNA含量的测定 。Based on the ability of nucleic acids to shift the association equilibrium of an ion association complex of alcian blue and tetra sulfonated aluminum phthalocyanine, thus leading to an increase in phthalocyanine fluorescence, a new method was suggested for the fluorimetric determination of nucleic acids. Investigations were carried out on the spectral characteristic, selection of the buffer system, effect of pH, influence of reaction time, the usage of reagents and interference of foreign substances. Under optimum conditions, the calibration curves for determinations of calf thymus deoxyribonucleic (DNA) (CTDNA), salmon DNA(SMDNA) and yeast ribonucleic acid (RNA) were linear over the range of 2.0～200 μg/L. The detection limits for CTDNA, SMDNA and RNA were 2.0 μg/L, 1.8 μg/L and 5.4 μg/L, respectively. This method was used to the determination of golden staplylococuus DNA and the result was in agreement with that achieved by an ultraviolet spectrophotometric method.

关 键 词：爱尔新蓝 缔合作用 四磺基铝酞菁 荧光染料 核酸 定量分析 光度法 

分 类 号：O657.3[理学—分析化学]