IL-13基因+2044G/A多态性与儿童哮喘风险和血清IL-13水平的相关性研究  

Association of IL-13 gene + 2044 G/A polymorphism to the risk of pediatric asthma and its relevancy to serum levels of IL-13

作　　者：丁宝栋 王明伟[1] 赵月华[2] 李海波[3] 魏守刚[1] 

机构地区：[1]潍坊医学院青州临床学院儿科,山东潍坊261053 [2]潍坊医学院附属医院儿科,山东潍坊261000 [3]潍坊市人民医院干细胞实验室,山东潍坊261041

出　　处：《临床儿科杂志》2016年第11期838-841,共4页Journal of Clinical Pediatrics

摘　　要：目的：探讨IL-13基因＋2044 G/A多态性与儿童哮喘发病风险及哮喘儿童血清IL-13水平的关系。方法采用PCR-RFLP和ELISA方法，对90例支气管哮喘患儿（哮喘组）和82例健康儿童（对照组）IL-13基因＋2044 G/A多态性和血清IL-13水平进行检测，计算基因型、等位基因频率及其所对应的血清IL-13水平。结果哮喘组和对照组IL-13＋2044G/A多态性3种基因型的分布差异有统计学意义（P＜0.001）；与GG基因型比较，GA基因型儿童哮喘风险增加3.52倍（95%CI：1.82-6.82），AA基因哮喘风险增加3.71倍（95%CI：1.06-12.97）。哮喘组A等位基因频率为36.1%，高于对照组的18.3%，差异有统计学意义（P＜0.001）。与G等位基因比较，A等位基因携带儿童哮喘风险增加2.53倍（95%CI：1.53-4.16）。哮喘组患儿血清IL-13水平为151.82（134.33-166.68）ng/L，对照组为79.00（58.74-111.09）ng/L，两组差异有统计学意义（P＜0.001）。哮喘组GG、GA和AA基因型的血清IL-13水平均分别高于对照组，差异有统计学意义（P均＜0.01）。哮喘组GG、GA和AA 3种基因型之间血清IL-13水平的差异也有统计学意义（P＜0.01），其中GA和AA基因型血清IL-13水平高于GG基因型。结论 IL-13基因＋2044 G/A多态性与支气管哮喘儿童血清IL-13水平具有关联性，且A等位基因可能造成了哮喘儿童血清高IL-13水平，携带A等位基因的儿童哮喘风险增加。Objective To investigate the relationship between IL-13 gene ＋ 2044G / A polymorphism to the risk of asthma in children and its relevancy of serum IL-13 levels.Methods Ninty cases of children with bronchial asthma （asthma group） and 82 healthy children （control group） were recruited. PCR-RFLP and ELISA were used to detectIL-13 gene 2044G〉A polymorphism and serum IL-13 levels. Genotype, allele frequencies and the corresponding serum IL-13 level were calculated.χ^2 test andt-test were used to compare the genotype, allele frequency, and serum IL-13 level between the two groups.Results The distribution ofIL-13 gene 2044G〉A polymorphism showed signiifcant difference between the two groups. Compared with AA genotype and A allele, children with GG genotype and G allele were associated with an increased risk of bronchial asthma, and theOR value were 3.706 （95%CI：1.059~12.976） （P〈0.001） and 2.525 （95%CI：1.532~4.159） （P〈0.001）. The level of serum IL-13 is higher in asthma group than in control group （Z= 7.899,P〈0.001）, and the serum IL-13 level of GA and AA in asthma group is higher than that in children with GG genotype （Z= 5.555,P〈0.001;Z= 3.541,P〈0.001;Z= 2.777, P〈0.05）.Conclusion There is association betweenIL-13 2044G〉A polymorphism and serum IL-13 level in children＇ with bronchial asthma. And A allele maybe associated with higher serum IL-13 level in children with asthma.

关 键 词：IL-13基因多态性 血清IL-13 哮喘 儿童 

分 类 号：R725.6[医药卫生—儿科]