热激对玉米大斑病菌Stste12基因沉默突变体发育的影响  

Effects of Heat Shock Treatment on Development of Stste12 Silencing Mutant of Setosphaeria turcica

作　　者：张运峰[1] 

机构地区：[1]唐山师范学院生命科学系,河北唐山063000

出　　处：《河南农业科学》2016年第11期71-75,共5页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31271997);河北省自然科学基金项目(C2014105067);河北省留学人员科技活动项目(C2015005009);唐山师范学院科学研究基金项目(2014E04;2013A03)

摘　　要：为了探明温度升高对玉米大斑病菌Stste12基因沉默突变体生长发育、基因表达水平等方面的影响,对玉米大斑病菌野生型菌株Wt01-23和Stste12基因沉默突变体StRNAi9-10同时进行热激处理,比较2个菌株菌落形态、菌丝形态、菌落生长速度及分生孢子产量方面的差异,并通过半定量PCR技术检测热激处理对Stste12基因表达的影响。结果显示,热激处理提高了突变体StRNAi9-10菌落的生长速度,其菌落形态和生长速度接近Wt01-23菌株,但对Wt01-23菌落的生长速度和形态无显著影响;半定量PCR检测显示,StRNAi9-10在热激处理后Stste12基因表达量增加,且随着温度的升高而增加,而Wt01-23菌株的Stste12基因表达量未发生显著变化。以上结果表明,热激处理恢复了玉米大斑病菌RNAi突变体的生理特征,具有抑制RNAi的作用。In order to explore the effect of temperature rise on the growth and Ststel2 expression of Setosphaeria turcica Ststel2 silencing mutants, the effects of heat shock treatment on the colony and mycelium morphology, colony growth speed, and spore formation of wild-type strain Wt01-23 and Ststel2 silencing mutant StRNAi9-10 were analyzed in this paper, and semi-quantitative PCR was performed to examine the effect of heat shock treatment on the expression of Ststel2. The results demonstrated that heat shock treatment had no significant impact on the colony growth speed and morphology of Wt01-23,but significantly accelerated the growth of StRNAi9-10, which had similar growth speed and colony morphology to WtOl -23 under the heat shock treatment. Ststel2 mRNA level in StRNAi9-10 was significantly up-regulated after heat shock treatment in a temperature-dependent manner, but not in Wt01-23. These data showed that heat shock treatment could inhibit RNAi silencing, and restore the physiological characteristics of Setosphaeria turcica RNAi mutant.

关 键 词：热激 玉米大斑病菌 发育 Stste12 半定量PCR 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治] Q935[农业科学—植物保护]