Quantification of albumin in urine using preconcentration and near-infrared diffuse reflectance spectroscopy  

作　　者：Xiao-Xu Ma Cui-Cui Wang Wen-Sheng Cai Xue-Guang Shao 

机构地区：[1]Research Center for Analytical Sciences,College of Chemistry,Nankai University,Tianjin 3000TI,China [2]Tianjin Key Laboratory of Biosensing and Molecular Recognition,Tianjin 300071,China [3]State Key Laboratory of Medicinal Chemical Biology,Tianjin 300071,China [4]Collaborative Innovation Center of Chemical Science and Engineering,Tianjin 300071,China

出　　处：《Chinese Chemical Letters》2016年第10期1597-1601,共5页中国化学快报（英文版）

基　　金：National Natural Science Foundation of China(No.21475068);MOE Innovation Team(No.IRT13022)of China

摘　　要：Urinary albumin is an important diagnostic and prognostic marker for cardiorenal disease, Recent studies have shown that elevation of albumin excretion even in normal concentration range is associated with increased cardiorenal risk. Therefore, accurate measurement of urinary albumin in normal concentration range is necessary for clinical diagnosis. In this work, thiourea-functionalized silica nanoparticles are prepared and used for preconcentration of albumin in urine. The adsorbent with the analyte was then used for near-infrared diffuse reflectance spectroscopy measurement directly and partial least squares model was established for quantitative prediction. Forty samples were taken as calibration set for establishing PLS model and 17 samples were used for validation of the method. The correlation coefficient and the root mean squared error of cross validation is 0.9986 and 0.43, respectively. Residual predictive deviation value of the model is as high as 18.8. The recoveries of the 17 validation samples in the concentration range of 3.39-24.39 mg/L are between 95.9%-113.1%. Therefore. the method may urovide a candidate method to auantifv albumin excretion in urine_Urinary albumin is an important diagnostic and prognostic marker for cardiorenal disease, Recent studies have shown that elevation of albumin excretion even in normal concentration range is associated with increased cardiorenal risk. Therefore, accurate measurement of urinary albumin in normal concentration range is necessary for clinical diagnosis. In this work, thiourea-functionalized silica nanoparticles are prepared and used for preconcentration of albumin in urine. The adsorbent with the analyte was then used for near-infrared diffuse reflectance spectroscopy measurement directly and partial least squares model was established for quantitative prediction. Forty samples were taken as calibration set for establishing PLS model and 17 samples were used for validation of the method. The correlation coefficient and the root mean squared error of cross validation is 0.9986 and 0.43, respectively. Residual predictive deviation value of the model is as high as 18.8. The recoveries of the 17 validation samples in the concentration range of 3.39-24.39 mg/L are between 95.9%-113.1%. Therefore. the method may urovide a candidate method to auantifv albumin excretion in urine_

关 键 词：Near-infrared diffuse reflectancespectroscopyQuantitative determinationMultivariate calibrationUrinary albuminThiourea-functionalized silicananoparticles 

分 类 号：R446.12[医药卫生—诊断学]