人内皮祖细胞移植对脊髓损伤大鼠功能恢复的研究  

Effect of human endothelial progenitor cell transplantation treatment on the functional recovery of rats with spinal cord injury

作　　者：刘铮[1] 康雪峰[1] 李晶[1] 黄媛[1] 戴宁[1] 张钊[1] 刘振中[1] Liu Zheng Kaag Xuefeng Li Jing Huang Yuan Dai Ning Zhang Zhao Liu Zhenzhong(Department Orthopaedics ,Heilongjiang Provincal Hospital,Harbin ,Heilongjaing 150036, China)

机构地区：[1]黑龙江省医院骨一科,黑龙江省哈尔滨150036

出　　处：《中国基层医药》2016年第21期3222-3225,共4页Chinese Journal of Primary Medicine and Pharmacy

基　　金：黑龙江省卫生和计划生育委员会科研课题重点项目（2014-137）

摘　　要：目的：观察人内皮祖细胞（hEPCs）移植对脊髓损伤（SCI）大鼠功能恢复的影响。方法24只SD 大鼠均先用快尖刀片将脊髓 T10节段完全横断完成动物模型制作，操作过程中死亡5只。后将19只脊髓横断大鼠随机分为实验组（10只）和对照组（9只）。实验组采用损伤脊髓处注射 hEPCs（human Endothelial Progenitor Cells）悬液7．5μL，对照组损伤脊髓邻近区注射等量 DMEM（Dulbecco minimum essential medium）培养液，术后每天腹腔注射免疫抑制剂环抱素 A，在术后1、2、4、6、8周进行 Basso Beatlie Bresnahan（BBB）评分来记录 SCI 大鼠双下肢神经功能恢复情况。术后8周处死大鼠，取出横断脊髓进行免疫组织化学和原位杂交技术，检测移植的 hEPCs 在大鼠横断脊髓内的存活情况。结果 SCI 模型制作后2周内，实验组和对照组的BBB 评分差异均无统计学意义（t ＝0．61、0．69，均 P ＞0．05）。术后第4周开始，实验组的 BBB 评分[（1．67±0．71）分]大于对照组的（1．11±0．60）分，差异有统计学意义（t ＝3．16，P ＜0．05）。第8周时分别为实验组（4．00±1．41）分和对照组（1．44±0．89）分，差异有统计学意义（t ＝5．384，P ＜0．05）。SCI 4、6、8周，实验组后肢功能恢复明显好于对照组，差异均有统计学意义（P ＜0．05）。原位杂交和免疫组化检测显示移植的 hEPCs 不仅能在宿主体内存活而且部分 hEPCs 嵌合到 SCI 后大鼠的脊髓及其血管系统。结论 hEPCs 移植后，能够在SCI 后大鼠脊髓内存活、并分化为血管内皮细胞，并且促进脊髓神经功能的恢复。Objective To observe the effects of human EP cells （hEPCs）transplantation on the function recovery of rats with spinal cord injury.Methods 24 SD rats （5 ones died in the process of operation）were firstly completed transection of T10 segment of spinal cord by using quick knife slices to complete animal model.19 rats after spinal cord transection were randomly divided into：the experimental group （10 cases）and the control group （9 cases）. The experimental group with spinal cord injury was injected 7.5μL suspension of hEPCs （human Endothelial Progeni-tor Cells）.Adjacent area of spinal cord injury in control group was injected equal amount DMEM（Dulbecco minimum essential medium.Postoperative intraperitoneal injection of cyclosporine A,an immunosuppressant,was administrated daily.At postoperative 1,2,4,6,8 weeks Basso Beatlie Bresnahan （BBB）score were administrated to record the neural function recovery of lower limbs in SCI rats.The transection spinal cord were removed from rats 8 weeks after operation to detect transplanted survival hEPCs in transection spinal cord of ratsby immunohistochemical and hybridization technique. Results 2 weeks after SCI model established,the BBB scores in the experimental group and the control group showed no statistically significant difference（t =0.61,0.69,all P 〉0.05）.4 weeks postoperatively,the BBB score of the experimental group （1.67 -0.71）points was more than that in the control group （1.11 -0.60）,the difference was statistically significant（t =3.16,P 〈0.05）.Untill 8 weeks scores were respectively the （4.00 -1.41）of the experimental group and the （1.44 -0.89）of the control group,the difference was statistically significant（t =5.384,P 〈0.05）. Finally it was concluded that 4,6,8 weeks after SCI,hind limbs function of the experimental group was significantly better than that of the control group,the difference had statistical significance （P 〈0.05）.Hybridization and immuno-histochemical detection showed that the tran

关 键 词：脊髓损伤 内皮细胞 细胞移植 

分 类 号：R651.2[医药卫生—外科学]