地塞米松预处理及治疗对ISO致小鼠急性心肌损伤影响的比较研究  

A comparative study on the effects of dexamethasone pretreatment and treatment on acute myocardial injury induced by isoproterenol in mice

作　　者：张严高[1] 余磊[2] 王建莉[2] 尹戴佳佳 朱闽湘[3] 

机构地区：[1]中国人民解放军南京军区杭州疗养院疗养一科,浙江杭州310007 [2]浙江大学免疫研究所,浙江杭州310058 [3]中国人民解放军第117医院神经外科,浙江杭州310013

出　　处：《河北医科大学学报》2016年第10期1157-1161,共5页Journal of Hebei Medical University

摘　　要：目的观察地塞米松(dexamethasone,DEX)对异丙肾上腺素(isoprotereno,ISO)致小鼠急性心肌缺血损伤的影响并探讨其作用机制。方法将16只昆明种小鼠随机分成正常对照(control,CON)组4只、ISO组4只、地塞米松预处理(dexamethasone pretreatment,DEX-pre)组4只、DEX组4只。CON组、ISO组首次处理前30min给予等量0.9%氯化钠注射液腹腔注射预处理。CON组给予等量0.9%氯化钠注射液腹腔注射处理。ISO组给予5mg/kg ISO,每日腹腔注射1次,连续3d。DEX-pre组在首次ISO注射前30min给予DEX 1.25mg/kg腹腔注射,余同ISO组。DEX组在第2天、第3天ISO注射30min后给予DEX 1.25mg/kg腹腔注射,余同ISO组。在HE染色光镜下观察心肌组织的病理学改变;酶联免疫吸附测定法检测血清肿瘤坏死因子α(tumor necrosis factor alpha,TNF-α)的变化;流式细胞分析检测脾脏T细胞亚群(杀伤性T细胞即CD4+T细胞、抑制性T细胞即CD8+T细胞、激活的杀伤性T细胞即CD4+CD69+T细胞、激活的抑制性T细胞即CD8+CD69+T细胞)变化。结果 1与ISO组比较,DEX-pre组心肌损伤得到明显改善(P<0.05),DEX组无明显变化(P>0.05)。2DEX组TNF-α含量明显高于CON组、ISO组和DEX-pre组,差异有统计学意义(P<0.05)。3DEX-pre组CD4+T细胞、CD4+CD69+T细胞、CD8+CD69+T细胞与其他3组差异有统计学意义(P<0.05),其他3组之间差异无统计学意义(P>0.05);DEX-pre组CD8+T细胞低于其他3组,DEX组低于CON组与ISO组,差异均有统计学意义(P<0.05)。结论 DEX预处理对ISO致小鼠急性心肌缺血损伤的心肌具有明显保护作用,而损伤后给予DEX治疗则无保护作用。可能机制与DEX预处理导致免疫功能改变及炎性细胞因子改变相关,确切机制有待进一步研究。Objective To investigate the potential protective effect of glucoeorticoid receptor agonist dexamethasone on isoproterenol （ISO）-induced myocardial injury and the possible mechanism. Methods The sixteen mice were randomly divided into control（CON） group（n=4）, ISO group （n= 4）, dexamethasone pretreatment （DEX-pre） group （n =4） arid dexamethasone treatment（DEX） group（n=4）. The mice in CON group and ISO group were given the same amount of 0.9% sodium chloride and DEX-pre group given dexamethasone （1.25 mg/kg） injection by intraperitoneal injection as a pretreatment at 30 minutes before treatment. CON group was given the same amount of 0. 9% sodium chloride injection by intraperitoneal injection ISO group, DEX-pre group and DEX group received 5 mg/kg isoproterenol hydrochloride, daily intraperitoneal injection of ltime, for 3 consecutive days. At the second and third day, dexamethasone（1.25 mg/kg） was given after ISO injected for 30 minutes in DEX group. Myocardial tissue injury was assessed by HE staining. Flow cytometry was adopted to detect the expression of CD4＋ T, CD8＋ T, CD4＋ CD69＋T, CD8＋ CD69＋- T cells in spleen. Results Compared with ISO group, the degree of myocardial injury were greatly improved（P〈（0. 05） the degree of myocardial injury wasn＇t significantly difference in DEX group （P〈 0.05）. （2） The expression of TNF-a in serum in DEX group was significantly difference to the other group（P〈 0.05）. （3）The expression Of CD4＋ T, CD4＋ CD69＋ T, CD8＋ CD69＋ T cells in DEX-lbre group was significantly difference to the other group（P〈0.05）. The expression of CD8＋ T cells in-DEX-pre group was significantly reduced to the other group（P〈0.05）. The expression of CD8＋ T Cells in DEX group was significantly reduced to the control group and ISO group（P〈0. 05）. Conclusion DEX pretreatment has protective effect against ISO-induced myocardial injury. But dexamethasone treatment don＇t have protective effect afte

关 键 词：心肌缺血 地塞米松 异丙肾上腺素 

分 类 号：R452.2[医药卫生—治疗学]