Population divergence of aggregation pheromone responses in Ips subelongatus in northeastern China  

作　　者：Da-Feng Chen Ye-Jing Li Qing-He Zhang Su-Fang Zhang Hong-Bin Wang Zhen Zhang Li-Lin Zhao Xiang-Bo Kong 

机构地区：[1]Key Laboratory of Forest Protection of State Forestry Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, China [2]State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China [3]Sterling International, Inc., Spokane, Washington, USA

出　　处：《Insect Science》2016年第5期728-738,共11页昆虫科学（英文版）

摘　　要：The Asian larch bark beetle, Ips subelongatus, is considered to be the major pest of larch within its natural range. We investigated the electrophysiological and behavioral characteristics as well as mitochondrial DNA cytochrome oxidase subunit I sequences of L subelongatus from 13 geographic populations throughout northeastern China in order to explore population divergence of aggregation pheromone responses and the extent of potential genetic divergence. Electrophysiological analyses showed that antennae of I. sube-longatus from all the six tested populations responded strongly to （S）-（-）-ipsenol （100% detection; 0.35-0.73 mV） in gas chromatography （GC）-electroantennographic detection （EAD） analyses, while its antipode, （R）-（＋）-ipsenol was antennally inactive. I. subelon- gatus populations varied in their responses to （R）-（-）- and （S）-（＋）-ipsdienol in GC-EAD analyses. Behavioral bioassays demonstrated that （S）-（-）-ipsenol alone was significantly attractive at all the tested sites, supporting its status as a key pheromone component of L subelongatus, whereas （S）-（＋）-ipsdienol was inactive alone. Adding （S）-（＋）-ipsdienol to （S）-（-）-ipsenol did not have any effect on the trap catches from some populations in Inner Mongolia. However, （S）-（＋）-ipsdienol showed a strong synergistic effect on （S）-（-）- ipsenol from several populations in Jilin and Liaoning Provinces, and a weak synergistic effect from some transition populations in Heilongjiang Province. Furthermore, 27 mito- chondrial haplotypes were found among the 13 populations （intraspecific nucleotide di- vergence, 0.1%-1.1%）. Analyses of molecular variance and haplotype networks indicated that different geographic populations have developed some genetic variation but did not form completely independent groups. From an applied point of view, a universal synthetic binary blend ofracemic ipsenol and （S）-（＋）-ipsdienol might have a potential for monitoriThe Asian larch bark beetle, Ips subelongatus, is considered to be the major pest of larch within its natural range. We investigated the electrophysiological and behavioral characteristics as well as mitochondrial DNA cytochrome oxidase subunit I sequences of L subelongatus from 13 geographic populations throughout northeastern China in order to explore population divergence of aggregation pheromone responses and the extent of potential genetic divergence. Electrophysiological analyses showed that antennae of I. sube-longatus from all the six tested populations responded strongly to （S）-（-）-ipsenol （100% detection; 0.35-0.73 mV） in gas chromatography （GC）-electroantennographic detection （EAD） analyses, while its antipode, （R）-（＋）-ipsenol was antennally inactive. I. subelon- gatus populations varied in their responses to （R）-（-）- and （S）-（＋）-ipsdienol in GC-EAD analyses. Behavioral bioassays demonstrated that （S）-（-）-ipsenol alone was significantly attractive at all the tested sites, supporting its status as a key pheromone component of L subelongatus, whereas （S）-（＋）-ipsdienol was inactive alone. Adding （S）-（＋）-ipsdienol to （S）-（-）-ipsenol did not have any effect on the trap catches from some populations in Inner Mongolia. However, （S）-（＋）-ipsdienol showed a strong synergistic effect on （S）-（-）- ipsenol from several populations in Jilin and Liaoning Provinces, and a weak synergistic effect from some transition populations in Heilongjiang Province. Furthermore, 27 mito- chondrial haplotypes were found among the 13 populations （intraspecific nucleotide di- vergence, 0.1%-1.1%）. Analyses of molecular variance and haplotype networks indicated that different geographic populations have developed some genetic variation but did not form completely independent groups. From an applied point of view, a universal synthetic binary blend ofracemic ipsenol and （S）-（＋）-ipsdienol might have a potential for monitori

关 键 词：aggregation pheromone GC-EAD field trapping Ips subelongatus mtDNA-COI population divergence 

分 类 号：Q[生物学]