乙肝病毒DNA和病毒标志物检测在出入境检疫人群中的应用  

Application of hepatitis B viral DNA and serum markers detections in the entry-exit quarantined population

作　　者：史玲莉[1] 李云[1] 闫冀焕[1] 李薇[1] 罗明[2] 华娜[3] SHI Ling-li LI Yun YAN Ji-huan LI Wei LUO Ming HUA Na(Healthcare Center ofHebeiEntry-Exitln- spection and Quarantine Bureau, Shijiazhuang 050091, China)

机构地区：[1]河北出入境检验检疫局保健中心,石家庄050091 [2]北京市疾病预防控制中心 [3]河北国际旅行卫生保健中心唐山分中心

出　　处：《国际病毒学杂志》2016年第5期318-320,共3页International Journal of Virology

基　　金：河北检验检疫局科研自主立项计划项目（HE2013K019）

摘　　要：目的 探讨出入境检疫人群中乙肝病毒DNA （HBV-DNA）与乙型肝炎患者血清病毒标志物（HBV-M）的相关性,为出入境检疫人群的乙型肝炎的传染性疾病监测和评估提供方法学依据.方法 选择2015年1月-2015年10月在本中心接受出入境检疫的人群,采用ELISA法检测HBV-M,对表面抗原阳性的血清采用荧光定量PCR法检测HBV-DNA,比较两种方法的相关性.结果 大三阳[HBsAg（＋）＋HBeAg（＋）＋HBcAb（＋）]与小三阳[HBsAg（＋）＋HBeAb（＋）＋HBcAb（＋）]模式的HBV-DNA阳性率分别98.65％和68.00％,两者有统计学差异.结论 从出入境检疫人群疾病监测的角度,HBeAg阳性,可判定其具有传染性,而对于HBsAg（＋）而HBeAg（-）的体检者,应进一步检测HBV-DNA以判断其是否具有传染性.Objective To investigate the relationship between Hepatitis B viral DNA （HBV-DNA） and serum viral markers （HBV-M） in the entry-exit quarantine population,so as to provide a methodological basis for the study of the infectious disease monitoring and assessment of hepatitis B in the quarantine population.Methods From January to October in 2015,the quarantined population were studied and the HBV-M was detected by ELISA.The serum HBsAg positive persons were selected as research subjects.HBV-DNA was detected by fluorescent quantitative PCR assay.The results of both methods were compared and the relationship was analyzed.Results The positive rates of HBV-DNA were 98.65% and 68.00% in thc HBsAg,HBeAg,and HBcAb positive mode and the HBsAg,HBeAb and HBcAb positive mode,respectively.The difference between both modes were statistically significant.Conclusions If HBeAg was positive,the serum can be considered to be contagious in the disease surveillance of the entry and exit quarantined people.In case of HBsAg （＋） and HBeAg （-）,the HBV-DNA should be further examined to determine the infectiousness.

关 键 词：乙肝病毒DNA 乙肝病毒标志物 出入境检疫人群 监测 

分 类 号：R512.620.4[医药卫生—内科学]