流动注射荧光分光光度法测定DNA的研究  

Determination of DNA by Fluorimetry with Flow Injection

作　　者：方光荣[1] 宋功武[1] 李玲[1] 詹红菊[2] 李襄宏 

机构地区：[1]湖北大学分析测试中心,湖北武汉430062 [2]湖北大学化学与材料学院,96级本科毕业生湖北武汉430062

出　　处：《光谱学与光谱分析》2002年第4期631-633,共3页Spectroscopy and Spectral Analysis

基　　金：中国分析测试基金资助课题

摘　　要：本文研究了中药有效成分盐酸小蘖碱与DNA的荧光光谱性质 ,并应用流动注射技术测定DNA的浓度 ,该法线性范围 0～ 12 μg·mL-1 ,检出限为 ( 3σ) 7 3ng·mL-1 ,相对标准偏差≤ 3 1% ,应用于合成样品的测定 。A highly sensitive fluorimetry determination of DNA is developed in this paper The maximum fluorescence intensity was produced in the pH 3 05,with the maximum excitation and emission wavelength of 362 and 531 nm,respectively Under the optimum conditions ,the fluorescence intensity was proportional to the concencentration of DNA The linear range for calf thymus DNA is of 0-120 mg·L -1 .The limit of determination was 0 07 mg·L -1 When the proposed method was used to determine DNA in the presence of some coexisting substances, a satisfactory result was obtained

关 键 词：流动注射 荧光分光光度法 测定 DNA 小蘖碱 荧光光谱 中药 相互作用 药理 分析 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]