早期激素性股骨头坏死过程中细胞色素c的作用  

Effects of cytochrome c on early steroid-induced femoral head necrosis

作　　者：吉浩宇[1] 王小龙[2] 

机构地区：[1]内蒙古医科大学附属医院骨科,内蒙古自治区呼和浩特市010050 [2]内蒙古医科大学第二附属医院手足外科,内蒙古自治区呼和浩特市010030

出　　处：《中国组织工程研究》2016年第42期6252-6258,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81360224)~~

摘　　要：背景:研究表明骨细胞凋亡与激素性股骨头坏死的发病机制密切相关,而细胞色素c的释放在细胞凋亡过程中起着非常重要的作用。目的:观察细胞色素c在早期激素性股骨头坏死的作用。方法:将24只成年健康5月龄新西兰雄性兔随机分成模型组与对照组,每组12只,模型组采用臀肌注射激素联合耳缘静脉注射马血清的方法建立早期激素性股骨头坏死模型,对照组耳缘静脉注射等量生理盐水,分别于造模第2,4,6周在光学显微镜下观察双侧股骨头组织病理学改变,并计算空骨陷窝率,应用TUNEL法检测骨细胞凋亡,并计算凋亡指数;应用免疫组织化学染色测定细胞色素c,并计算细胞色素c阳性表达率。结果与结论:(1)空骨陷窝率及凋亡指数:实验成功建立了早期激素性股骨头坏死兔模型,于对照组相比,模型组造模后各时间点的空骨陷窝率、凋亡指数及骨细胞细胞色素c阳性表达率均明显升高(P<0.05或P<0.01);(2)相关性分析:模型组各时间点的细胞凋亡指数与空骨陷窝率呈明显正相关(r=0.856,P<0.01),且模型组各时间点的细胞色素c阳性表达率与细胞凋亡指数同样呈明显正相关(r=0.824,P<0.01)。(3)结果证实:细胞色素c参与的骨细胞凋亡可能在激素性股骨头坏死中起重要作用,且骨细胞的细胞色素c阳性表达率与兔激素性股骨头坏死的发生呈显著正相关。BACKGROUND：Studies have shown that apoptosis is closely related to the pathogenesis of steroid-induced femoral head necrosis. The release of cytochrome c plays a very important role in the process of apoptosis. OBJECTIVE：To observe the effects of cytochrome c on early steroid-induced femoral head necrosis. METHODS：Twenty-four healthy adult male New Zealand rabbits at 5 months old were randomly divided into model group and control group （n=12 per group）. Models of early steroid-induced femoral head necrosis were established by intragluteal injection of hormone combined with ear vein injection of horse serum. In the control group, rabbits were given ear vein injection of the same amount of physiological saline. At 2, 4 and 6 weeks after model establishment, histopathological changes of bilateral femoral head were observed by optical microscope, and the ratio of empty lacuna was calculated. Apoptosis of osteocytes was determined by TUNEL assay, and apoptotic index was calculated. Immunohistochemical method was used to determine cytochrome c and to calculate cytochrome c-positive expression rate. RESULTS AND CONCLUSION：（1） The ratio of empty lacuna and apoptotic index：The model of early steroid-induced femoral head necrosis was successful y established in the experiment. Compared with the control group, ratio of empty lacuna, apoptotic index and expression rate of cytochrome c in osteocytes were significantly higher in the model group （P〈0.05 or P〈0.01）. （2） Correlation analysis：Ratio of empty lacuna was significantly positively associated with apoptotic index at various time points in the model group （r=0.856, P〈0.01）. Expression rate of cytochrome c was significantly positively associated with apoptotic index at various time points in the model group （r=0.824, P〈0.01）. （3） These findings confirm that cytochrome c-involved apoptosis of osteocytes may play an important role in steroid-induced femoral head necrosis. Expression rate of cytochrome c in osteocytes is rem

关 键 词：组织工程 细胞凋亡 模型 动物 组织构建 骨组织工程 激素性股骨头坏死 细胞色素C 早期 动物模型 凋亡指数 空骨陷窝 骨细胞:国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]