机构地区:[1]牡丹江医学院附属红旗医院,黑龙江省牡丹江市157011
出 处:《中国组织工程研究》2016年第42期6317-6323,共7页Chinese Journal of Tissue Engineering Research
基 金:牡丹江市科学技术计划项目;攻关类(Z2015s0045)~~
摘 要:背景:在组织相容性、免疫排斥反应及修复后瘢痕影响程度等方面,去细胞异体神经移植修复周围神经缺损更接近自体神经。目前,已经有透明质酸应用于自体周围神经修复的研究,尚无透明质酸应用于异体神经移植修复周围神经损伤的报道。目的:探讨透明质酸在去细胞同种异体神经移植修复大鼠坐骨神经缺损中对吻合口瘢痕形成的影响。方法:36只SD大鼠随机分为3组,每组12只。3组大鼠均选取左后肢手术,手术将坐骨神经锐性切断后造成10 mm神经缺损。实验组异体神经移植后在两端吻合口应用透明质酸,对照组行单纯异体神经移植手术,自体神经移植组将自体神经切断后远近端倒置吻合。术后观察近端吻合口的愈合情况,评估近端吻合口的瘢痕成分。结果与结论:(1)大体观察:大鼠皮肤,肌肉筋膜愈合组间无差别,周围组织粘连情况比较,实验组优于对照组(P<0.05);(2)Masson染色:各组神经外膜均可见胶原沉积,实验组神经外膜胶原纤维排列整齐有序,胶原纤维量略少;对照组大量胶原纤维成堆积状,排列紊乱;自体神经移植组神经外膜胶原纤维较多,胶原纤维排列较整齐,但胶原纤维较稀疏;(3)Ⅰ,Ⅲ型胶原免疫组化灰度值:Ⅰ型胶原灰度值实验组高于对照组(P<0.05),Ⅲ型胶原灰度值实验组低于对照组(P<0.05),Ⅰ型+Ⅲ型胶原灰度值实验组、对照组及自体神经移植组比较差异无显著性意义(P>0.05);(4)结果提示,透明质酸在周围神经损伤修复过程中对Ⅰ,Ⅲ型胶原沉积具有调控作用,可增加Ⅲ型胶原沉积,减少Ⅰ型胶原沉积,从而减少瘢痕形成。BACKGROUND:In terms of the histocompatibility, immune rejection and scar formation after repair, acel ular nerve al ograft is closer to autologous nerve cel s. At present, hyaluronic acid has been applied for autologous peripheral nerve repair;however, research on the nerve al ograft is rarely reported. OBJECTIVE:To explore the effect of hyaluronic acid on the anastomotic scar in acel ular nerve al ograft repair of rat sciatic nerve defect. METHODS:Thirty-six Sprague-Dawley rats were randomly divided into three groups (n=12 per group). The rat model of nerve defect of 10 mm was established by cutting the sciatic nerve of the left hind leg and then given nerve al ograft combined with the injection of hyaluronic acid at anastomosis (experimental group), only nerve al ograft (control group) and autologous nerve graft (nerve autograft group), respectively. Afterwards, the healing of the proximal anastomosis was observed and scar components were assessed. RESULTS AND CONCLUSION:Gross observations showed that the rat skin and muscle fascia had no significant differences in healing among groups, while the surrounding tissue adhesion in the experimental group was milder than that in the control group (P〈0.05). Masson staining found that col agen deposition in the epinerium could be observed in each group. In the experimental group, a smal amount of col agen fibers arranged orderly in the epineurium;in the control group numerous col agen fibers accumulated and arranged irregularly;in the nerve autograft group, sparse epineurial col agen fibers appeared in an order arrangement. The gray value of col agen type I in the experimental group was higher than that in the control group (P〈0.05), while the gray value of col agen type III was lower than that in the control group (P〈0.05). No significant differences were found in the sum gray values of col agen type I and III among groups (P〉0.05). These findings indicate that in the peripheral nerve repair, hyaluronic acid abrogates
关 键 词:透明质酸 坐骨神经 瘢痕 胶原Ⅰ型 胶原Ⅲ型 组织构建 组织工程 坐骨神经缺损 去细胞异体神经 Ⅰ型胶原 Ⅲ型胶原 神经移植 吻合口 愈合
分 类 号:R318[医药卫生—生物医学工程]
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