神经细胞分泌因子促进骨骼肌细胞生长  

Neuron-secreted factors promote the growth of skeletal muscle cells

作　　者：蔡明轩[1] 王美辰[1] 徐治成 颜南[2] 孟欣雨 王正东[3] 

机构地区：[1]沈阳医学院基础医学院,辽宁省沈阳市110034 [2]沈阳医学院康复教研室,辽宁省沈阳市110034 [3]沈阳医学院解剖学教研室,辽宁省沈阳市110034

出　　处：《中国组织工程研究》2016年第42期6324-6329,共6页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省大学生创新创业训练计划项目(20151161);沈阳医学院大学生科研项目(20150801)~~

摘　　要：背景:周围神经损伤可引起支配肌肉萎缩,目前尚无明确的方法治疗肌肉萎缩。肌细胞是实现肌肉收缩功能的结构单位,体外培养的骨骼肌细胞可用于失神经肌肉萎缩相关研究。目的:观察神经元分泌因子对体外骨骼肌细胞的生长的促进作用。方法:将体外原代培养的骨骼肌细胞分为2组,实验组加神经细胞分泌因子培养,对照组加普通培养液培养,用苏木精-伊红染色、免疫组织化学染色检测神经元分泌因子作用后骨骼肌细胞数目及α肌动蛋白表达的变化。结果与结论:(1)骨骼肌细胞数量、骨骼肌细胞肌动蛋白的表达:实验组骨骼肌细胞数量、骨骼肌细胞α肌动蛋白的表达均多于对照组(P<0.05);(2)结果说明:神经元分泌因子有促进大鼠骨骼肌细胞生长的作用,可能具有治疗失神经肌肉萎缩的作用。BACKGROUND：There is no effective treatment for muscle atrophy caused by peripheral nerve damage. Skeletal muscle cel s, a structural unit of muscle contraction, can be used for studies on muscle atrophy when cultured in vitro. OBJECTIVE：To investigate the promotion effect of neuron-secreted factors on the growth of skeletal muscle cel s in vitro. METHODS：Skeletal muscle cel s primary cultured in vitro were divided into two groups：experimental group with neuron-secreted factors, and control group with common culture medium, respectively. Afterwards, the number of skeletal muscle cel s and expression level of alpha actin were detected by hematoxylin-eosin staining and immunohistochemical staining, respectively. RESULTS AND CONCLUSION：The number of skeletal muscle cel s and expression level of alpha actin in the experimental group were significantly higher than those in the control group （P〈0.05）. In conclusion, neuron-secreted factors have the ability of promoting the growth of skeletal muscle cel s and may be helpful for denervated muscle atrophy.

关 键 词：组织工程 卫星细胞 骨骼肌 辅肌动蛋白 神经元 细胞 培养的 组织构建 骨骼肌细胞 细胞培养 Α肌动蛋白 促进 分泌因子 肌肉萎缩 肌卫星细胞 周围神经损伤 

分 类 号：R318[医药卫生—生物医学工程]