具有微弧氧化-硅烷-褪黑素复合膜层钛合金种植体的体内骨结合  

In vivo osseointegration of micro-arc oxidation-silane-melatonin-covered titanium alloy implant

作　　者：李德超[1] 刘惠萍[1] 李慕勤[1] 刘苗[1] 

机构地区：[1]佳木斯大学黑龙江省高校生物医学材料重点实验室,黑龙江省佳木斯市154007

出　　处：《中国组织工程研究》2016年第43期6438-6444,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(31370979);黑龙江省高校创新团队建设计划项目(2012TD010);佳木斯大学协同创新中心(2011xtcx2016-02)~~

摘　　要：背景:钛合金种植体与骨界面结合的强度不理想,延长了种植体与周围骨组织的愈合时间。目的:为进一步提高钛合金口腔种植体的早期愈合能力,对钛合金种植体进行表面改性,观察其骨结合能力。方法:分别制备具有微弧氧化膜层的钛合金种植体(设为A组)、微弧氧化-硅烷复合膜层的钛合金种植体(设为B组)与微弧氧化-硅烷-褪黑素复合膜层的钛合金种植体(设为C组)。将3种种植体分别置入新西兰大白兔下颌骨中,置入后2,4,6周,采用锥形束CT、荧光显微镜、扭力实验检测种植体-骨界面早期成骨情况。结果与结论:(1)锥形束CT检查结果:置入后6周,A组种植体与下颌骨结合不紧密;C组种植体与下颌骨结合紧密,骨结合最好;B组骨结合情况介于两者之间;(2)荧光显微镜观察结果:3组种植体置入4周的荧光最强,6周时稍有减弱,C组不同时间点的荧光强度高于A、B组;(3)扭力实验结果:随着置入时间的延长,3组种植体骨结合力逐渐增强,C组不同时间点的骨结合力高于A、B组(P<0.05);(4)结果表明:具有微弧氧化-硅烷-褪黑素复合膜层的钛合金种植体具有较高的骨结合能力活性,可促进早期成骨。BACKGROUND： Imperfect strength of titanium alloy implant-bone interface delays bone healing. OBJECTIVE： In order to further improve the early healing ability of titanium dental implants, to make the surface modification of titanium alloy implant and observe the osseointegration ability of the implant. METHODS： Titanium alloy implants were coated with micro-arc oxidation （group A）, micro-arc oxidation-silane （group B）, and micro-arc oxidation-silane-melatonin （group C） coatings, respectively. Three kinds of implants were placed into the mandible of New Zealand white rabbits, and 2, 4, 6 weeks after implantation, implant-bone interface osseointegration was observed using cone-beam CT, fluorescence microscopy, and torsion test. RESULTS AND CONCLUSION; （1） Cone-beam CT examination： 6 weeks after implantation, the osseointegration of the implant with the mandible achieved the best results in the group C, followed by group B and group A. （2） Fluorescence microscope observation： the implants in the three groups exhibited the strongest fluorescence at 4 weeks and slightly weakened at 6 weeks, and the fluorescence intensity was higher in the group C than the other two groups. （3） Torsion test： with implantation time prolonged, the implant-bone osseointegration activity gradually increased in the three groups, while it was still highest in the group C （P 〈 0.05）. In summary, our results show that the titanium alloy implant coated with micro-arc oxidation-silane-melatonin composite coating has higher osseointegration activity and promotes bone formation.

关 键 词：牙种植体 骨结合 组织工程 生物材料 口腔生物材料 钛种植体 种植体-骨界面 偶联剂 褪黑素 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]