等离子体处理聚氨酯-胶原-硫酸软骨素复合材料的制备及性能评价  

A plasma-modified polyurethane-collagen-chondroitin sulfate composite material: preparation and performance

作　　者：玄光善[1] 穆兰兰 孙桐[1] 李青 

机构地区：[1]青岛科技大学药学系,山东省青岛市266000 [2]青岛中皓生物工程有限公司,山东省青岛市266000

出　　处：《中国组织工程研究》2016年第43期6451-6457,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:聚氨酯具有优异的机械性能和良好的生物相容性,但其疏水性不利于细胞黏附生长,故限制了聚氨酯在皮肤支架上的应用。目的:对聚氨酯支架材料进行改性,观察其形态结构,检测其亲水性、机械性能及细胞毒性。方法:通过静电纺丝方法制备聚氨酯支架材料,然后进行CO2、NH3等离子体处理,最后通过交联剂交联,与胶原蛋白及硫酸软骨素复合,制备等离子体处理聚氨酯-胶原-硫酸软骨素复合材料,设置为A组;设置3组对照,聚氨酯经静电纺丝后进行等离子体处理制备成支架材料后,浸泡在未加交联剂的胶原硫酸软骨素混合溶液中,取出风干,设为B组;聚氨酯经静电纺丝后进行等离子体处理,制备成支架材料,设为C组;将聚氨酯进行静电纺丝,制备成支架材料,设为D组。对比4组材料的形态结构、亲水性、机械性能及体外细胞毒性。结果与结论:等离子体处理聚氨酯-胶原-硫酸软骨素复合材料具有良好的纤维形貌、亲水性及机械性能,可促进细胞的黏附、增殖,无细胞毒性。BACKGROUND： Polyurethane has excellent mechanical properties and perfect biocompatibUity. But its hydrophobicity is not conductive to the adhesion and growth of cells, which limits its use as the scaffold of the skin. OBJECTIVE： To modify a polyurethane scaffold by plasma processing and crosslinking then to test its hydrophilic, mechanical properties and cytotoxicity in vitro. METHODS： Polyurethane porous materials were prepared into membranous materials by eletrospinning method and modified by CO2 and NH3 plasma. Then the plasma-modified polyurethane-collagenchondroitin sulfate composite materials were prepared by crosslinking with collagen type I and chondroitin sulfate at the same time, which was named Group A. Polyurethane porous materials which were electrospun and modified by plasma were soaked into a mixed solution of collagen and chondroitin sulfate without crosslinking agent. After being air-dryed, the materials were prepared, named Group B. Polyurethane porous materials which were electrospun and modified by plasma were named Group C. Electrospun polyurethane porous materials without other treatments were named Group D. Then the morphology, hydrophilicity, mechanical property and in vitro cytotoxicity of the four groups were compared. RESULTS AND CONCLUSION： The plasma-modified polyurethane-collagen-chondroitin sulfate composite materials enjoy excellent fiber morphology, hydrophilicity and mechanical properties. It can also promote cell adhesion, proliferation and have no cytotoxicity.

关 键 词：胶原 软骨素硫酸盐类 组织工程 生物材料 材料相容性 等离子体 聚氨酯 硫酸软骨素 复合材料 细胞毒性 生物相容性 

分 类 号：R318[医药卫生—生物医学工程]