小鼠跨区供血耳瓣choke区的组织学观察  

Histopathological Observation on a Cross-territory Flap Choke Area of Ear in Mouse

作　　者：温福利[1] 梁成[1] 庄跃宏[2] 吴剑威[1] 郑和平[1] 

机构地区：[1]南京军区福州总医院比较医学科,福州350025 [2]福建医科大学人体解剖与组织胚胎学系,福州350108

出　　处：《实验动物与比较医学》2016年第5期345-349,共5页Laboratory Animal and Comparative Medicine

基　　金：福建省自然科学基金资助项目（2015J01412）和南京军区医学科技创新重大专项（ZX30）

摘　　要：目的 观察小鼠跨区供血耳瓣模型血管组织学和形态学变化.方法 以小鼠右耳为实验组,用眼科剪从尾侧向头侧剪断小鼠耳基底部尾侧2/3,保留头侧1/3,建立耳前动静脉为蒂内含3个血管体和2个choke区的跨区供血耳瓣小鼠模型.左耳为对照组,仅作脱毛处理.在术后0d、1d、3d、5d、7d分别切取鼠耳置于体积分数10％甲醛固定,用于观察耳瓣choke Ⅰ区和choke Ⅱ区的组织学变化.结果 不同时间点choke Ⅰ区实验组血管管径均大于对照组（P＜0.01）;choke Ⅰ区和choke Ⅱ区静脉血管数量分别在3d和5d达到峰值,然后逐渐减少,这一趋势可能与耳瓣基底部静脉回流的建立有关.choke Ⅰ区和choke Ⅱ区动脉血管充盈度平均值分别为40.64％和38.85％,静脉血管充盈度平均值分别为57.02％和61.48％.结论 ①跨区耳瓣切取后,静脉扩张是被动扩张,而动脉扩张是主动增值;②跨区耳瓣切取后choke Ⅱ区扩张的choke血管数量及扩张度均小于choke Ⅰ区;③跨区耳瓣切取后,血管管径变化对皮瓣存活起积极作用.Objective To observe the histological and morphological changes of mouse＇s crossterritory ear flap model of vascular. Methods The right-ear of mice as experimental group, the caudal of the mouse ears were cut for 2/3 with eye scissors from caudal to cephalic direction, ear＇s flap based on a vascular pedicle but crossed three vascular territories was created.The left-ear as the control group, just clearing the hair. The ear of mice were surgically cut in 0 d, 1 d, 3 d, 5 d, 7 d respectively postoperation, and fixed in 10% formaldehyde for histological observation of the changes of choke I area and choke II area in the ear＇s flap. Results At different time points, the average value of choke I area of blood vessels in the experimental group was larger than that of the other groups （P〈0.01）. The amount of venous blood vessels in choke I and choke II area respectively reached peak value at 3 d and 5 d, and then decreased gradually, and this trend may be related to the establishment of the venous reflux in the basal part of the ear flap.In choke I and choke II area, the mean values of arterial and venous blood vessel filling degree were 38.85%, 40.64% and 57.02%, 61.48%, respectively. Conclusions ① After establishment of extended flap, the dilation of veins seemed to passive, whereas the dilation of arteries seemed to active; ② The number of the choke vessels between the dynamic and potential territories that are involved in dilation and extent of the dilation are much smaller than that of the choke vessels between the anatomic and dynamic territories; ③ The change of vascular diameter has positive effect on the survival of skin flap.

关 键 词：小鼠 耳瓣 组织学观察 choke血管 

分 类 号：R641[医药卫生—外科学] R-332[医药卫生—临床医学]