高脂饲料诱发C57BL／6小鼠肥胖性II型糖尿病  

High-fat Diet Induced Type 2 Diabetes Mellitus and Obesity in C57BL/6 Mice

作　　者：梁永俊[1] 张鹏[1] 王月倩[1] 高黎黎[1] 曹婷[1] 乔正东[1] 

机构地区：[1]上海市浦东医院,复旦大学附属浦东医院,上海201399

出　　处：《实验动物与比较医学》2016年第5期361-364,共4页Laboratory Animal and Comparative Medicine

基　　金：上海市浦东新区重点学科群（PWZxq2014-08）、上海市浦江人才计划（14PJ1407800）、上海市浦东新区卫生系统学科带头人（PWRd2013-08）

摘　　要：目的 通过饲喂高脂饲料诱发C57BL/6小鼠Ⅱ型糖尿病（T2DM）,探讨不良饮食对T2DM以及肥胖症的影响.方法 以C57BL/6小鼠为模型,饲喂脂肪供热60％的高脂饲料12周,检测小鼠高脂饲料饲喂前后空腹血糖（FBG）及体质量,并通过口服糖耐量试验（OGTT）反应胰岛β细胞功能受损情况和机体对血糖调节能力的改变.结果 与对照组比较,从第4周开始C57BL/6小鼠的空腹血糖显著上调（P＜0.001）,同时体质量稳步增长直至第12周形成肥胖症（P＜0.001）,分析显示空腹血糖与体质量的变化存在显著相关性（P＜0.001）.口服糖耐量试验结果表明,高脂饲料饲喂12周后C57BL/6小鼠血糖调节能力严重受损（P＜0.001）.结论 C57BL/6小鼠高脂饲料饲喂12周后可成功建立肥胖型T2DM模型.该模型可良好再现因不良饮食习惯所引起T2DM的发生,为糖尿病的研究提供有力工具.Objective To investigate the effect of high fat diet on Type 2 dialbetes mellitus （T2DM） and obesity, the model of T2DM was established by using prolonged high-fat diet （HFD） feeding on C57BL/6 mice. Methods C57BL/6 mice were fed with 60F10S diet for 12 weeks, the fasting bloodglucose （FBG） and animal body weight were monitored at six different time points. Moreover, oral glucose tolerance test （OGTT） was carried out to detect the blood glucose regulation ability and reflect the β cells dysfunction. Results Compare to the control group, the FBG had significantly increased from fourth week （P〈0.001）, while body weight grew steadily until the 12th week to formation of obesity （P〈0.001）. Correlation analysis reveals that the changes of FBG and body weight were correlated （P〈0.001）. After twelve weeks HFD feeding, the area under the curve of the OGTT （in mmol · h-1 · L-1） was extraordinarily enlarged which means that the mice had poorer ability of blood glucose regulation （P〈0.001）. Conclusion Obese models of T2DM can be successfully induced by 12 weeks＇ HFD feeding in C57BL/6 mice. As the simulation of high-fat diet in modem life, this model can reproduces the T2DM caused by diet disorders, and provides a powerful tool for the study of diabetes.

关 键 词：C57BL/6小鼠 高脂饲料 II型糖尿病模型 

分 类 号：R587.1[医药卫生—内分泌] R-332[医药卫生—内科学]