Development of a low-noise cryogenic atomic force microscope for high resolution imaging of large biological complexes  

作　　者：Ling Qin Jinjin Zhang Jielin Sun Daniel M. Czajkowsky Zhifeng Shao 

机构地区：[1]Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China [2]Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Jiading Campus, Shanghai 201800, China [3]Bio-ID Center, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China

出　　处：《Acta Biochimica et Biophysica Sinica》2016年第9期859-861,共3页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China （Nos. 91129000, 11374207, 31370750, 21273148, 11405250 and 11074168）, MOST （No. 2014YQ090709）, the Science and Technology Commission of Shanghai Municipality （No. 15142201200）, SJTU （No. YG2012MS58）, and the K.C. Wong Education Foundation （H.K.）.

摘　　要：There is presently a significant demand for instruments capable of determining the structures of large biological complexes. While room temperature atomic force microscopy （RT-AFM） has provided direct sub-nanometer resolution images of individual proteins, the resolution in images of larger biological assemblies is rather poor at least partly due to the inherent softness of these samples [1,2]. We previously developed a unique cryogenic atomic force microscope （cryo-AFM） specifically for biological samples, which can image at temperatures as low as 77 K [3].There is presently a significant demand for instruments capable of determining the structures of large biological complexes. While room temperature atomic force microscopy （RT-AFM） has provided direct sub-nanometer resolution images of individual proteins, the resolution in images of larger biological assemblies is rather poor at least partly due to the inherent softness of these samples [1,2]. We previously developed a unique cryogenic atomic force microscope （cryo-AFM） specifically for biological samples, which can image at temperatures as low as 77 K [3].

分 类 号：Q[生物学]