油茶产黄酮内生真菌的发酵工艺优化  

Optimization of Fermentation Conditions of Flavonoids-producing Strain of Camellia oleifera

作　　者：张婷[1] 

机构地区：[1]荆楚理工学院生物工程学院,湖北荆门448000

出　　处：《现代农业科技》2016年第20期260-261,263,共3页Modern Agricultural Science and Technology

基　　金：湖北省教育厅科研项目(Q20134301);校级科研基金项目(ZR201403)

摘　　要：在单因素试验的基础上,采用正交试验法对油茶产黄酮内生真菌的发酵条件进行研究,结果表明,油茶产黄酮内生真菌YUH-2的最适发酵条件为:培养基起始p H值为7,接种量为5%,培养温度为28℃,转速170 r/min,其黄酮产量为3.18 mg/100 m L,比未优化时高出42%。The fermentation conditions of flavonoids producing strain of Cemellia oleifera were studied using orthogonal test, based on the single factor experments.The results showed that the optimum factors were as follow：the optimal intial pH value was 7,inoculation amount was 5% ,the culture temperature was 28 ℃ ,fermentation speed was 170 r/min.The flavonoids yield was 3.18 mg/100 mL,improved by 42% ,compared with the original state of strain Y-UH-2.

关 键 词：黄酮产量 油茶 植物内生菌 单因素优化 正交优化 

分 类 号：S794.4[农业科学—林木遗传育种]