中哈边境阿拉山口口岸亚洲璃眼蜱首次检测到Q热立克次体核酸  

The first detection of Coxiella burnetii DNA from Hyalomma asiaticum at Alataw pass,China-Kazakhstan border area

作　　者：罗丹[1] 尹小平[2] 王安东[2,3] 田延河[2] 梁臻[2] 巴特[2] 张江国[2] 

机构地区：[1]石河子大学医学院,新疆石河子832000 [2]阿拉山口出入境检验检疫局,新疆阿拉山口833418 [3]新疆石河子大学动物科技学院,新疆石河子832000

出　　处：《疾病监测》2016年第10期814-816,共3页Disease Surveillance

基　　金：国家质检总局科技计划项目(No.2016lk264)~~

摘　　要：目的对新疆阿拉山口口岸地区蜱类进行Q热立克次体核酸检测。方法采集蜱样本并进行形态学和分子生物学鉴定。聚合酶链反应扩增Q热com1基因,通过Blast软件比对分析测序产物,利用Mega 6.0软件构建分子遗传进化树。结果共采集253只蜱,其中优势蜱种为亚洲璃眼蜱,蜱类的形态学鉴定与分子生物学鉴定结果一致。阿拉山口口岸地区蜱携带Q热立克次体核酸平均阳性率为16.21%(41/253),亚洲璃眼蜱阳性率为22.65%(41/181),血红扇头蜱、短垫血蜱和边缘革蜱均为阴性,亚洲璃眼蜱显著高于其他蜱种。序列分析显示与Coxiella burnetii Cb175_Guyana(HG825990,圭亚那)进化关系较近,核苷酸同源性为99%。结论阿拉山口口岸地区亚洲璃眼蜱在16S rRNA和COⅠ基因序列间存在多样性,并首次在中哈边境地区亚洲璃眼蜱中检测到Q热立克次体核酸。Objective To understand the Coxiella burnetii infection and genotype status in ticks at Alataw pass in China- Kazakhstan border area in Xinjiang. Methods The ticks were collected by drag-flag at Alataw pass. Coxiella burnetii was detected by using coml gene primers with PCR. The PCR products were sequenced and analyzed with BLAST and phylogenetic tree was constructed with software Mega 6.0. Results A total of 253 ticks were collected at Alatawpass and the predominant tick species was H. asiaticum. And the morphological identification result was consistent with the molecular identification result. The average positive rate of Coxiella burnetii was 16. 21% （41/253）. The positive rate in H. asiaticum was higher than that in D. marginatus, H. erinacei and R. sanguineus. The positive rate of CoxieUa burnetii in H. asiaticum was 22. 65% （41/181）. The sequencing analysis indicated that the Coxiella burnetii detected in H. asiaticum at Alataw pass shared 99% homology with the Coxiella burnetii Cb175_Guyana （HG825990）. Conclusion In this study, the I-1. asiaticum at Alataw pass showed genetic diversity at 16S rRNA and in CO I gene sequence, and it was the first time to detect Coxiellaburnetii in H. asiaticum at Alataw pass in China-Kazakhstan border area.

关 键 词：Q热立克次体 com1基因 亚洲璃眼蜱 中哈边境地区 

分 类 号：R376.4[医药卫生—病原生物学]