Enterovirus 71病毒引起重症手足口病患儿外周血调节性T细胞、辅助性T细胞17及相关细胞因子的表达  

Expression of Treg cells, TH17 cells and related cytokines in peripheral blood of patients with severe hand-foot-mouth disease caused by Enterovirus 71 virus

作　　者：冯爱东[1] 杨淑岭[1] 阎纳新[1] 刘云燕[1] 吴春晓[2] 杨莉[1] 

机构地区：[1]河北省石家庄市第五医院肝病研究所,050021 [2]河北省中医院,050021

出　　处：《中国综合临床》2016年第10期876-880,共5页Clinical Medicine of China

基　　金：河北省中医药管理局科研计划项目（2015045）

摘　　要：目的：研究Enterovirus 71（ EV71）病毒引起的重症手足口病患儿感染初期外周血中辅助性T细胞17（ Th17）与调节性T细胞（ Treg细胞）及相关细胞因子的水平变化。方法收集2013年石家庄市第五医院住院重症手足口病患儿35例，重型组27例，危重型组8例，采用流式细胞术检测患儿和31名正常儿童外周血中（ CD3＋CD8-IL-17＋） Th17及（ CD4＋CD25＋Foxp3＋） Treg细胞表达率，采用ELISA方法检测血清中IL-6、IL-17、IL-10、IL-23、TGF-β1的水平。结果重症手足口患儿各组及正常对照组，Treg细胞表达率、Treg／Th17比率及TGF-β1、IL-10水平比较差异均有统计学意义且与对照组相比明显降低，（F值分别为23．23、30．88、10．766、11．680，P均＜0．05），Th17细胞表达率及血清中IL-6、IL-17、IL-23比较差异亦均有统计学意义且与对照组相比明显升高（ F 值分别为646．81、5．904、10．557、3．490， P 均＜0．05）。结论 EV71病毒引起的重症手足口患儿感染初期外周血Treg／Th17细胞及相关细胞因子的水平出现变化，通过减少Treg细胞，增加TH17细胞，扩大炎症反应，引发并发症。Objective To investigate the changes of Treg cells,Th17 cells and related cytokines in pe-ripheral blood of patients with severe hand-foot-mouth disease（ HFMD） in the initial stage of infection caused by Enterovirus 71 virus. Methods Thirty-five cases of severe HFMD children in the Fifth Hospital of Shijiazhuang from May 2013 to December 2013 were selected as the research subjects,including 27 cases of severe formed group and 8 cases of danger severe formed group. The frequencies of Th17 cells （ CD3＋CD8-IL-17＋） and Treg cells（ CD4＋CD25＋Foxp3＋） in peripheral blood were detected by flow cytometry in patients with severe HFMD and 31 healthy children（control group）. Moreover the serum levels of interleukin 6（IL-6）,interleukin 17（IL-17） ,interleukin 10（ IL-10） ,interleukin 23（ IL-23） ,and human transforming growth factorβ1 （ TGF-β1） were measured by ELISA. Results As compared with those in control group,the frequencies of Treg cells,ratio of Treg/Th17,serum levels of TGF-β1, IL-10 in severe HFMD groups were significantly decreased （ F=23. 23, 30. 88,10. 766,11. 680 respectively;P〈0. 05） . However the frequency of Th17 cells,serum levels of IL-6,IL-17,IL-23 in severe HFMD groups were significantly increased（ F=646. 81,5. 904,10. 557,3. 490 respectively;P〈0. 05） . Conclusion The changes of Treg and Thl7 cells and related cytokines appear in peripheral blood of patients with HFMD in the initial stage of infection caused by Enterovirus 71 virus. The frequency of Treg cell is decreased,however,the frequency of Thl7 cells frequency is increased,as a result,which may spread inflammato-ry reaction and cause complication.

关 键 词：手足口病 TREG细胞 TH17细胞 细胞因子 

分 类 号：R725.1[医药卫生—儿科]