酶联免疫吸附法与免疫印迹法检测谷氨酸脱羧酶抗体的比较  

Comparison of enzyme-linked immunosorbent assay(ELISA) and immunoblotting test(IBT) in glutamic acid decarboxylase antibody(GADAb)testing

作　　者：王睿智[1] 陈志江[1] 周晖[2] 邝建[1] 裴剑浩[1] 

机构地区：[1]广东省人民医院(广东省医学科学院)内分泌科,广州510080 [2]广东省人民医院(广东省医学科学院)检验科,广州510080

出　　处：《中国糖尿病杂志》2016年第11期983-985,共3页Chinese Journal of Diabetes

基　　金：国家科技支撑计划课题(2013BAI09B12);广东省药学会2型糖尿病用药研究基金(2012C14)

摘　　要：目的探讨ELISA和免疫印迹(IBT)法检测GADAb的差异。方法分别采用糖尿病自身抗体免疫印迹试剂盒、Euroimmune ELISA试剂盒和放射配体结合(RBA)法检测118例新诊断糖尿病患者血清中的GADAb,比较3种方法的检测结果,以及IBT和Euroimmune ELISA试剂盒与RBA的一致性。结果 GADAb的阳性检出率:RBA(13.6%)=ELISA(13.6%)>IBT(5.1%)。与RBA法的一致性:ELISA(98.3%.Kappa值为0.928)>IBT(91.5%,Kappa值为0.509)。以RBA作为诊断标准,ELISA的曲线下面积(AUC)为0.968。Euroimmun ELISA试剂盒检测GADAb的浓度与RBA的GADAb-Index呈正相关(r=0.985)。结论 Euroimmune ELISA试剂盒检测GADAb的效果优于IBT,与RBA相当。Objective To investigatethe difference between enzyme-linked imrnunosorbent assay （ELISA） and irnmunoblotting test （IBT） in glutamic acid decarbpxylase antibody （GADAb） testing. Methods A total of 118 patients with newly diagnosed diabetes were enrolled in this study. GADAb was measured by ELISA（Euroimmune ELISA kit）,IBT and radioligand binding assay（RBA）respectively in all the subjects. All the results were compared and the consistency of Euroimrnune ELISA kit and IBT with RBA were evaluated. Results Positive rate of GADAb： RBA （13.6%） = ELISA （13.6%） 〉 IBT （5. 1%）. Compared with RBA, concordance ratio of 2 assays with RBA： ELISA（98. 3%, Kappa value 0. 928）〉IBT（91.5%, Kappa value 0. 509）. The area under the receiver operating curve （ROC）of Euroimmun ELISA kit is 0. 964, regarding RBA as the standard diagnostic criteria. The GADAb concentration by Euroimrnun ELISA kit is positively correlated with RBA-GAGAb index（r= 0. 985）. Conclusion The performance of ELISA（Euroimrnune ELISA kit） is comparable to RBA and better than IBT in GADAb testing.

关 键 词：放射配体结合法 酶联免疫吸附法 免疫印迹法 谷氨酸脱羧酶 抗体 

分 类 号：R587.1[医药卫生—内分泌] R446.6[医药卫生—内科学]