氯麻滴鼻剂含量测定方法的建立  

Establishment of Content Determination Method for Chloramphenicol and Ephedrine Nasal Drop

作　　者：耿铮[1] 车慧[1] 徐超斗[1] 

机构地区：[1]总后卫生部药品仪器检验所,北京100071

出　　处：《中国药师》2016年第11期2153-2154,共2页China Pharmacist

基　　金：军队医疗机构制剂标准提高科研专项课题(编号:13ZJZ17)

摘　　要：目的:研究氯麻滴鼻剂含量测定方法。方法:色谱柱为SPOLAR C_(18)柱(250 mm’4.6 mm,5(m);流动相为0.02 mol·L^(-1)磷酸二氢钾溶液-甲醇(67∶33)(磷酸调p H至3.5);检测波长为220 nm。结果:氯霉素在10.43~156.42μg·ml^(-1)浓度范围内线性良好(r=0.999 9),平均回收率为99.0%,RSD为0.4%(n=9);盐酸麻黄碱在41.10~616.56μg·ml^(-1)浓度范围内线性良好(r=0.999 7),平均回收率为98.6%,RSD为0.4%(n=9)。结论:该方法操作简便,准确可靠,可作为氯麻滴鼻剂的含量测定方法。Objective： To study the determination method for chloramphenicol and ephedrine nasal drop. Methods： The column was SPOLAR C18 （250 mm×4.6mm,5μm）. The mobile phase was 0.02 mol·L^-1 potassium dihydrogen phosphate-methanol （67 ： 33, adjusting the pH value to 3.5 with phosphoric acid）. The detection wavelength was set at 220 nm. Results： The linearity of chloramphenicol was obtained within the range of 10.43-156.42μg·ml^-1（r = 0. 999 9）. The average recovery was 99.0% and RSD was 0.4% （n = 9）. The linearity of ephedrine hydrochloride was promising within the range of 41.10-616.56μg·ml^-1 （r = 0. 999 7 ）. The average recovery was 98.6% and RSD was 0.4% （ n = 9）. Conclusion： The method is convenient, accurate and reliable, and suitable for the determination of ehloramphenicol and ephedrine nasal drop.

关 键 词：氯麻滴鼻剂 氯霉素 盐酸麻黄碱 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]