大鲵虹彩病毒贵州分离株MCP基因的克隆与B细胞表位预测  

Cloning of the MCP gene of the isolate of giant salamander iridovirus from Guizhou and the prediction of B-cell epitopes in the MCP gene

作　　者：王芳 康超 余波[2] 李永霞 周思旋[2] 

机构地区：[1]贵州省生物研究所,贵阳550009 [2]贵州省畜牧兽医研究所,贵阳550005

出　　处：《黑龙江畜牧兽医》2016年第11期14-16,22,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：贵州省科学技术基金计划项目(黔科合J字[2014]2115号);贵州省科技厅农业攻关项目(黔科合NZ字[2012]3023号);2014年省级财政渔业发展(大鲵产业)专项资金项目

摘　　要：为了研究大鲵虹彩病毒(GSIV)MCP基因以及预测其B细胞表位,试验采用PCR方法扩增大鲵虹彩病毒MCP基因,采用生物学软件对大鲵虹彩病毒MCP基因的氨基酸序列进行B细胞表位预测分析。结果表明:扩增产物大小为1 392 bp,将该序列与Gen Bank中大鲵虹彩病毒MCP基因中的中国分离株核苷酸相似性为99.8%。MCP蛋白有多个抗原表位位点,抗原性指数较高,而这些区域可能是B细胞抗原表位区域。说明预测的结构、亲水性、柔性区域和抗原指数可用于大鲵虹彩病毒亚单位疫苗和分子诊断试剂的研究。To study MCP gene of giant salamander iridovirus （GSIV） and predict its B -cell epitopes, a PCR assay was used to amplify the MCP gene of GSIV in the test. Biological software was used for predictive analysis of B - cell epitopes in the amino acid sequence of the MCP gene of GSIV. The results showed that the size of amplified product was 1 392 bp were amplified, and the sequence shared 99.8% nucleotide similarities with that of Chinese isolates published in GenBank. MCP protein had multiple antigenic - epitope sites with higher antigenic indexes, while these areas might be B - cell epitope regions. The results indicate that the predicted structure, hydrophilicity, flexible regions, and antigenic indexes can be used for the study on the subunit vaccine against giant salamander iridovirus and molecular diagnostic reagents.

关 键 词：大鲵虹彩病毒(GSIV) MCP基因 B细胞表位预测 氨基酸序列 抗原指数 

分 类 号：Q785[生物学—分子生物学] Q786