芳香叠氮化合物对牛血清白蛋白的化学修饰  

The Modification of Bovine Serum Albumin with Aryl Azide

作　　者：苗俊玲 李赛[2] 刘晓蕾[2] 刘孝波[2] 

机构地区：[1]吉林省松原市长岭县医院,吉林松原131500 [2]中国科学院成都有机化学研究所,四川成都610041

出　　处：《合成化学》2002年第4期348-350,共3页Chinese Journal of Synthetic Chemistry

摘　　要：合成了一种芳香叠氮化合物的活性酯 ,利用该酯催化 4-叠氮基苯甲酸的羧基与牛血清白蛋白的氨基之间的反应形成酰胺键。研究了不同反应时间内 BSA的修饰率及其构象的变化。红外、紫外光谱证实了修饰的结果 ,荧光光谱检测了 BSA构象变化。A kind of photoactive aryl azide 4 azidobenzoic acid was modified to bovine serum albumin(BSA). This modified BSA could be photochemically immobilized onto polymer materials surface. The ratio of modification of BSA and the conformational change of BSA due to the reaction with active ester were discussed. The experimental results showed that the ratio of modification of BSA increased with the reaction time. The IR data and UV absorption were given to confirm the modification. The synchronous fluorescence spectral verified the conformational change of BSA.

关 键 词：芳香叠氮化合物 牛血清白蛋白 化学修饰 催化 4-叠氮基苯甲酸 

分 类 号：Q51[生物学—生物化学]