革兰氏阳性菌非编码小RNA的研究进展  

Advances in Research on Small Non-coding RNAs of Gram-positive Bacteria

作　　者：张健[1] 吴昊[1] 李艳妮[1] 乔建军[1] 

机构地区：[1]天津大学化工学院系统生物工程教育部重点实验室天津化学化工协同创新中心合成生物学平台,天津300072

出　　处：《中国生物工程杂志》2016年第10期106-114,共9页China Biotechnology

基　　金：国家自然科学基金项目(31270142;31570049);国家科技支撑计划(2014BAD02B00)资助项目

摘　　要：sRNA(非编码小RNA)通过碱基配对的方式与靶mRNA结合,抑制或激活转录过程、调节蛋白质的表达,以核酸的形式发挥其生物学功能。随着RNA深度测序(RNAseq)技术、生物信息学预测以及实验分析手段的日渐发展和完善,数以百计的sRNA被发现并得到验证。作为转录后调控因子,sRNA因在诸多生理过程中起到了关键的调节作用而得到了广泛的关注。以革兰氏阳性菌为切入点,总结了近年来sRNA的筛选、鉴定和功能研究等方面取得的进展,梳理分析了sRNA调控与毒力因子、群体感应、铁代谢和双组分系统等之间的内在联系,并展望了sRNA未来的研究方向。Bacterial small non-coding RNAs（ sRNAs）,an emerging class of regulatory RNAs,interact with their mRNA targets by base-pairing to inhibit or activate their translation,regulate expression of protein,and possess various biological functions. Recently, with the development of RNA-seq, bioinformatics and experimental techniques, hundreds of sRNAs have been explored in bacteria. As regulator factors at the posttranscriptional level,sRNAs have received extensive attention due to their important roles in biological processes. The sRNAs in gram-positive bacteria are focused on. The researches on screening,identification and functions of sRNA are summarized and the inherent relation between sRNAs and virulence factors,quorum sensing,iron metabolism as well as two-component system is elucidated and analyzed,whilst future study trends were proposed.

关 键 词：革兰氏阳性菌 非编码小RNA 调控因子 

分 类 号：Q752[生物学—分子生物学]