3株木糖氧化无色杆菌烈性噬菌体的最佳保存方法研究  

Optimal condition for preservation of three Achromobacter xylosoxidans bacteriophages

作　　者：黎尔纳[1] 马艳艳[2] 程伟伟[1] 蒋爱民[1] 袁静[3] 赵向娜[3] 

机构地区：[1]华南农业大学食品学院,广州510642 [2]河南科技学院食品学院,河南新乡453003 [3]军事医学科学院疾病预防控制所,传染病控制中心,北京100071

出　　处：《军事医学》2016年第10期814-818,共5页Military Medical Sciences

基　　金：国家自然科学基金资助项目(31200137)

摘　　要：目的寻找木糖氧化无色杆菌噬菌体简便高效的保存方法。方法将该噬菌体用不同的方法和温度保存,在不同时间点检测噬菌体的效价。结果与结论在-80℃、-20℃和4℃温度保存时,甘油和二甲基亚飒保存16个月后,phi Axp-1和phi Axp-2效价能稳定在初始的1010PFU/ml,phi Axp-3效价略微从1011下降到1010PFU/ml。使用氯仿在4℃保存16个月后,3株噬菌体的效价都略微下降,但优于其他保存温度(-80℃、-20℃、室温和37℃)。因此,这些都是保存木糖氧化无色杆菌噬菌体的合适方法。Objective To find simple and effective methods of preservation for Achromobacter xylosoxidans bacteriophages. Methods The plaque forming unit（ PFU） of surviving phages under different preservation conditions and temperatures at different time points was determined. Results and Conclusion The titers of phi Axp-1 and phi Axp-2 maintained at the initial 1010 PFU / ml,and that of phi Axp-3 decreased slightly from 1011 PFU / ml to 1010 PFU / ml 16 months after the three Achromobacter xylosoxidans bacteriophages were stored in glycerol and dimethyl sulfoxide at- 80℃,- 20℃ and4℃. When stored in chloroform at 4℃ for 16 months,the titers of all the three phages decreased slightly but were higher than at other temperatures（- 80℃,- 20℃,room temperature,and 37℃）. Thus these methods can effectively preserve Achromobacter xylosoxidans bacteriophages.

关 键 词：木糖氧化无色杆菌 噬菌体 保存 

分 类 号：R378[医药卫生—病原生物学]