青枯菌侵染不同抗病烟草品种的防御性酶活性及代谢组分差异分析  

Differences of defensive enzyme activities and metabolites between resistant and susceptible tobacco cultivars infected by Ralstonia solanacearum

作　　者：周星洋 张功营[1] 董丽红[1] 杨恩兰[1] 万树青[1] 

机构地区：[1]华南农业大学农学院/天然农药与化学生物学教育部重点实验室,广东广州510642

出　　处：《华南农业大学学报》2016年第3期73-81,共9页Journal of South China Agricultural University

基　　金：广东省烟草专卖局(公司)科技项目(粤烟[2009]17号)

摘　　要：【目的】为烟草抗病育种和抗性鉴定提供生化代谢指标。【方法】采用茎部注射法对抗病品种粤烟97和感病品种长脖黄接种青枯雷尔菌Ralstonia solanacearum,并采用比色法和GC-MS法分别进行相关防御性酶活性及代谢物质的测定。【结果】抗病品种粤烟97的PAL、SOD、POD、PPO活性,接菌组与对照组均分别高于感病品种长脖黄的接菌组与对照组;抗病品种粤烟97和感病品种长脖黄分别在接菌后第7天和第5天,PAL活性高于对照组,其余时间酶活性接菌组均低于对照组;抗病品种粤烟97和感病品种长脖黄在受青枯雷尔菌侵染前期,SOD、POD、PPO酶活性均提高,随着时间延长酶活性均低于对照组。POD和PPO同工酶凝胶电泳表明:抗病品种粤烟97同工酶类型多于感病品种长脖黄;接菌后第3天,粤烟97的同工酶谱带宽度与色度增强,而长脖黄无增强现象。说明抗病品种能快速应对外界刺激,加速相关抗病物质的形成。代谢物检测表明:抗病品种粤烟97接菌组中肌肉肌醇、烟碱、苹果酸、L-苏氨酸等物质的相对质量分数高于对照组,而感病品种长脖黄接菌组中这些物质均低于对照组,反映品种间抗青枯病能力的强弱可能与上述物质有关。【结论】烟草不同抗病品种叶片中PAL、SOD、POD、PPO酶活性的高低,以及上述代谢物质含量的变化,可作为反映烟草抗青枯病的生化指标。[ Objective ] In order to provide the biochemical disease resistance and resistance identification. [ Method （Changbohuang） tobacco cultivars were inoculated with and metabolic basis of tobacco breeding for ] Resistant （ Yueyan 97 ） and susceptible Ralstonia solanacearum by trunk injection. Defensive enzyme activities and metabolites were measured by using colorimetric method and GC-MS. [ Result ] The activities of phenylanine ammonia lyase （ PAL）, superoxide dismutase （ SOD）, peroxidase （POD） and polyphenol oxidase （PPO） in the resistant cuhivar ＇Yueyan 97＇ were higher than those in the susceptible cultivar ＇ Changbohuang＇ with the same inoculation treatment. The PAL activities of Yueyan 97 and Changbohuang were higher than that in control on the seventh and fifth days after inocula- tion, respectively. The PAL activities of both cuhivars were lower compared to control at any other time. For both cultivars, SOD, POD and PPO activities increased compared to control in the earlier days, but were lower compared to control during the later time period. The types of POD and PPO isozymes in the resistant cuhivar ＇ Yueyan 97＇ were higher than those in the susceptible cultivar ＇ Changbohuang＇. The width and color of isozyme bands of Yueyan 97 were enhanced at 3 d after inoculation, while those of Changbohuang were not enhanced. It suggested that the resistant cultivar could quickly respond to exter- nal stimuli and accelerate the formation of defense-related substances. Detection of metabolites showed that the relative contents of some substances in Yueyan 97 such as myo-inositol, nicotine, malic acid, and L-threonine were higher compared to control, but in Changbohuang were lower compared to control, suggesting that cuhivar differences in resistance against R. solanacearum might be related to these sub- stances. [ Conclusion ] These four defensive enzyme activities and the contents of the above metabolites could be used as biochemical indexes for evaluating tobacco resistance aga

关 键 词：烟草 青枯雷尔菌 防御性酶 酶活性 同工酶 代谢组分 

分 类 号：S432.42[农业科学—植物病理学]