硝磺草酮降解菌HZ-2制剂固定化载体材料性能的比较  

Pexformance comparison of different carrier materials for immobilization of mesotrione-degrading strain HZ-2

作　　者：张晶晶[1] 杨孟然 刘婕[1] 蒋刚彪[2] 钟国华[1] 

机构地区：[1]华南农业大学农学院,广东广州510642 [2]华南农业大学材料与能源学院,广东广州510642

出　　处：《华南农业大学学报》2016年第3期86-89,共4页Journal of South China Agricultural University

基　　金：广东省科技计划项目(2014B020206003)

摘　　要：【目的】研究改性蔗渣MSB、MSB-Mo、MSB-Cl对硝磺草酮降解菌HZ-2的降解能力的影响。【方法】以这3种改性蔗渣和活性炭为固定化载体,对固定化后菌制剂的降解性能进行研究。【结果】降解菌制剂培养48 h后,D600 nm均较高,表明MSB、MSB-Mo、MSB-Cl及活性炭均具备较强的固菌能力;培养3 d后,以改性蔗渣为载体的固菌制剂对LB培养基中硝磺草酮的降解率高于85%,显著优于活性炭,其中改性蔗渣MSB-Mo效果突出,降解率达96.35%。添加葡萄糖辅料7 d后,这3种改性蔗渣对土壤中硝磺草酮的降解率均高于99%。【结论】改性蔗渣MSB-Mo是固定降解菌株的最佳新型生物载体材料。[ Objective ] To evaluate the effect of modified bagasse （ MSB, MSB-Mo and MSB-CI） on the immobilization of mesotrione-degrading bacteria strain HZ-2. [ Method ] Three types of modified bagasse and activated carbon were selected as the carrier candidates for the bacterial immobilization, and the deg- radation abilities of immobilized composites were examined. [ Result ] After 48 hours culture, the high D600 n^values indicated that modified bagasse （MSB, MSB-Mo and MSB-CI） and activated carbon had strong abilities to immobilize bacteria. More than 85% of mesotrione in LB medium was degraded using modified bagasse as a carrier after 3 days of culture, which was higher than the results of activated car- bon. MSB-Mo composite had the best degradation performance with a degradation rate of 96. 35%. Moreover,mesotrione in soil was degraded over 99% after 7 days by adding glucose as support nutrient to the modified bagasse. [ Conclusion ] Modified bagassse MSB-Mo is the most available carrier material, which demonstrates the highest potential of being a new biological carrier material for immobilization of mesotri- one-degrading bacteria strain.

关 键 词：硝磺草酮 改性蔗渣 载体材料 降解能力 

分 类 号：S482.2[农业科学—农药学]