黄芩素对糖尿病肾病大鼠肾组织p38MAPK表达的影响  

Influence of baicalein on expression of p38 mitogen-activated protein kinase in the kidney of diabetic rats

作　　者：何日明[1] 门凌[1] 易无庸[1] 易铁钢[1] 李顺民[1] 杨曙东[1] 

机构地区：[1]广州中医药大学深圳附属医院肾病研究室,广东深圳518033

出　　处：《广东医学》2016年第20期3006-3009,共4页Guangdong Medical Journal

基　　金：广东省中医药局建设中医药强省科研课题(编号:20141243);广东省深圳市科技计划项目(编号:JCYJ20130329155553733;JCYJ20140408152909284)

摘　　要：目的探讨黄芩素对糖尿病肾病大鼠肾组织中p38MAPK、核因子-κB(NF-κB)p65表达的干预作用。方法将30只SD大鼠随机分为模型组、黄芩素干预组及正常对照组,每组10只,黄芩素干预组大鼠予黄芩素300 mg/(kg·d)灌胃,模型组予等体积0.5%羧甲基纤维素钠灌胃。用ELISA法测定24 h尿白蛋白量,免疫组化法检测p38MAPK、p-p38MAPK及NF-κB p65蛋白定位及表达。结果模型组大鼠24 h尿白蛋白量、p38MAPK、p-p38MAPK及NF-κB p65蛋白表达均明显高于正常对照组;与模型组相比,黄芩素干预组的上述指标均降低(均P<0.05)。结论黄芩素可能通过影响p38MAPK/NF-κB信号通路对糖尿病大鼠肾脏起保护作用。Objective To investigate the influence of baicalein on expression of p38 mitogen - activated protein kinase （p38MAPK） in the kidney of diabetic rats. Methods SD rats were randomly divided into model, baicalein intervention [300 mg/（kg . d） ] and normal control groups. The 24h urinary albumin was evaluated by ELISA. The expression and localization of p38MAPK, p -p38MAPK and NF - KB p65 were detected by immunohistochemistry. Results Compared with the normal control group, 24h urinary albumin （P 〈0.05）, and expression of p38MAPK, p - p3$MAPK and NF - κB p65 were significantly increased in the model group, which were significantly reversed by baicalein treatment （P 〈 0. 05）. Conclusion Baicalein can reduce the kidney injury in diabetic rats through inhibiting the p38MAPK/NF - κB signal pathway.

关 键 词：黄芩素 糖尿病肾病 P38丝裂原活化蛋白激酶 NF-κB P65 

分 类 号：R651.2[医药卫生—外科学]