脂肪源性干细胞对大鼠坐骨神经功能恢复的影响  

EFFECTS OF ADIPOSE DERIVED STEM CELLS ON FUNCTIONAL RECOVERY OF SCIATIC NERVE IN RATS

作　　者：付秀美[1] 王荣良 杨海艳 杨振江[1] 付文亮[1] 

机构地区：[1]承德医学院人体解剖学教研室,河北承德067000 [2]承德市妇幼保健院 [3]承德市中医院

出　　处：《承德医学院学报》2016年第6期451-453,共3页Journal of Chengde Medical University

基　　金：河北省教育厅基金项目(QN2014138);河北省重点发展学科"人体解剖与组织胚胎学"建设项目资助

摘　　要：目的:探讨脂肪源性干细胞(ADSCs)对大鼠坐骨神经功能恢复的影响。方法:将第4代ADSCs移植入脱细胞神经移植物(ANA)中,构建组织工程神经。大鼠随机分为正常对照组、培养基组和实验组,培养基组和实验组均建立坐骨神经损伤模型,然后用相应的组织工程神经桥接损伤神经的断端。术后6W和12W采用神经电生理记录仪检测各组大鼠坐骨神经传导速度和波幅。结果:术后6W、12W,实验组坐骨神经传导速度和波幅明显高于培养基组(P<0.05),但低于正常对照组(P<0.01);并且,实验组术后12W时的神经传导速度和波幅明显高于术后6W(P<0.05)。结论:ADSCs可增加坐骨神经传导速度和波幅,促进坐骨神经功能的恢复。Objeetive： To investigate the effects of adipose derived stem cells （ADSCs） on functional recovery of sciatic nerve in rats. Methods： The 4th generation ADSCs were transplanted into the acellular nerve allograR （ANA） to construct the tissue engineering nerves. The rats were randomly divided into normal control group, culture medium group and experimental group. The rats in culture medium group and experimental group were established sciatic nerve injury model, then corresponding tissue engineering nerves were used to bridge the injuried sciatic nerves. 6 and 12 weeks after operation, the sciatic nerve conduction velocity and amplitude were detected by nerve electrophysiology recorder. Results： The sciatic nerve conduction velocity and amplitude of rats in experimental group 6 weeks and 12 weeks after operation were obviously higher than culture medium group （P〈0.05）, but obviously lower than normal control group （P〈0.01 ）; Moreover, the sciatic nerve conduction velocity and amplitude of rats in experimental group 12 weeks after operation were obviously higher than that of rats 6 weeks after operation （P〈0.05）. Conclusion： ADSCs can increase the sciatic nerve conduction velocity and amplitude, so they could promote recovery of sciatic nerve function.

关 键 词：脂肪源性干细胞 大鼠 坐骨神经 神经传导速度 波幅 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]