黄芪注射液对脑缺血再灌注大鼠海马Bcl-2、Bax、Caspase-3表达的影响  

EFFECTS OF ASTRAGALUS INJECTION ON BCL-2, BAX AND CASPASE-3 EXPRESSION IN HIPPOCAMPUS OF CEREBRAL ISCHEMIA REPERFUSION RATS

作　　者：董雅洁[1] 高维娟[2] 卢锴锋[1] 谢亚芹[1] 朱炎杰[1] 

机构地区：[1]承德医学院,河北承德067000 [2]河北中医学院

出　　处：《承德医学院学报》2016年第6期453-455,共3页Journal of Chengde Medical University

基　　金：河北省科技支撑计划项目(13272504D);河北省教育厅青年基金项目(Q2012002);河北省高校重点学科建设项目资助(冀教高【2013】4号病理学与病理生理学)

摘　　要：目的:观察黄芪注射液对脑缺血再灌注大鼠海马Bcl-2、Bax、Caspase-3表达的影响。方法:雄性SD大鼠随机分为假手术组、脑缺血再灌注模型组、黄芪注射液溶剂组、黄芪注射液组,采用四血管阻断法建立脑缺血再灌注模型,分别采用免疫组化法和Western blot法检测海马Bcl-2、Bax、Caspase-3蛋白的表达。结果:与假手术组比,模型组Bcl-2、Bax、Caspase-3蛋白表达明显升高,Bcl-2/Bax明显降低(P<0.05);与模型组比,黄芪注射液组Bcl-2蛋白表达、Bcl-2/Bax明显升高,Bax、Caspase-3蛋白表达明显降低(P<0.05)。结论:黄芪注射液可通过升高Bcl-2的表达,降低Bax、Caspase-3的表达,抑制脑缺血再灌注大鼠海马神经元凋亡。Objective： To investigate the effects of astmgalus injection on Bcl-2, Bax and Caspase-3 expression in hiplx）campus of cerebral ischemia reperfusion rats. Methods： Male SD rats were randomly divided into sham-operation group, cerebral ischemia reperfusion model group, aslragalus injection solvent group and astragalus injection group. Four-vessel occlusion was used to establish cerebral ischemia reperfusion rats＇ model. was used to detect Bcl-2, Bax and Caspase-3 expression in hiippocampus. Results： Compared with sham-operation group, Bcl-2, Bax and Caspase-3 expression of rats in model group increased obviously, but Bcl-2/Bax decreased obviously （P 〈 0.05）. Compared with model group, Bcl-2 and Bcl-2/Bax of rots in astragalus injection group increased obviously, but Bax and Caspased-3 decreased obviously （P〈 0.05）. Condusions： Astragalus injection can inhibit neuronal apoptosis in of cerebral ischemia reperfusion rats by up regulating Bcl-2 expression and down regulating Bax, Caspase-3 expression.

关 键 词：脑缺血再灌注 黄芪注射液 Bcl-2 BAX CASPASE-3 

分 类 号：R285.5[医药卫生—中药学]