猪甲状腺球蛋白免疫诱导小鼠Graves病实验研究  

Experimental study on Graves' disease in mice models induced by porcine thyroglobulin

作　　者：李丽[1] 王鹏远 池莲祥[1] 廖春燕[1] 王敏琴[1] 叶韬[1] 钟丽娣 

机构地区：[1]深圳市宝安区人民医院内分泌科,广东深圳518100 [2]深圳市南山区蛇口人民医院口腔科,广东深圳518067

出　　处：《中华实用诊断与治疗杂志》2016年第11期1065-1067,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：2014年深圳市科技计划项目(JCYJ20140414112101551)

摘　　要：目的探讨猪甲状腺球蛋白(porcine thyroglobulin,pTg)免疫诱导C57BL6小鼠Graves病模型的效果及适宜剂量。方法 8周龄雌性C57BL6小鼠29只,随机分为对照组9只,免疫1组10只,免疫2组10只,对照组仅给予蒸馏水+弗氏佐剂,免疫1组和免疫2组分别给予pTg 25、50μg/鼠+弗氏佐剂进行免疫,10周龄时加强免疫1次,之后每周加强免疫1次,共8次。19周龄时,3组小鼠采用放射免疫法测定血清游离甲状腺素(free thyroxine,FT4)水平,采用ELISA法测定甲状腺刺激性抗体(thyroid stimulating antibody,TSAb)水平,并行甲状腺组织病理学检查。结果免疫2组小鼠血清FT4[(55.61±22.30)pmol/L]、TSAb[(2 546.76±606.06)μIU/L]水平明显高于对照组[(15.11±5.69)pmol/L、(1 805.18±213.48)μIU/L](P<0.05);免疫1组小鼠血清FT4[(24.74±17.40)pmol/L]、TSAb[(2 157.10±536.92)μIU/L]与对照组比较差异无统计学意义(P>0.05);免疫2组小鼠血清FT4水平高于免疫1组(P<0.05),TSAb水平与免疫1组比较差异无统计学意义(P>0.05);免疫1组4只和免疫2组7只小鼠甲状腺组织病理检查示甲状腺弥漫性肿大,甲状腺滤泡上皮细胞高度增加,且甲状腺未见明显淋巴细胞浸润;免疫2组Graves病成模率(70%)高于免疫1组(40%)(P<0.05);免疫2组TSAb与FT4呈明显正相关(r=0.863,P=0.006)。结论利用pTg诱导C57BL6小鼠Graves病模型可行,且50μg/鼠可能是最适宜的免疫抗原剂量。Objective To establish C57BL6 Graves＇ disease mice models induced by porcine thyroglobulin （pTg） and to monitor the effect and suitable dose of pTg. Methods Twenty-nine female C57BL6 mice aged 8 weeks old were divided into control group （n=9）, experimental 1 group （n= 10） and experimental 2 group （n= 10）. Control group was given distilled water and Freund adjuvant. Experimental 1 and 2 groups were immunized with 25 and 50 μg pTg plus Freund adjuvant, respectively, and received booster immunization at 10 weeks old. In the following 8 weeks, these mice were given booster immunization once a week. The serum free thyroxine （FT4） level was detected by radioimmunoassay, and thyroid stimulating antibody （TSAb） level was detected by ELISA technique in three groups and histopathological examination was done. Results The levels of serum FT4 （（55. 61 ± 22. 30） fmol/mL） and TSAb （（2 546. 76 ± 606.06） /μIU/L） in experimental 2 group were significantly higher than those in control group （（15.11±5.69） fmol/mL, （1 805. 18±213. 48） μIU/L） （P〈0.05）, and there were no significant differences between experimental 1 group （（24.74±17.40） fmol/mL, （2 157.10±536.92） μIU/L） and control group （P〉0.05）. The FT4 level was significantly higher in experimental 2 group than that in experimental 1 group （P〈0.05）, and there was no significant difference in TSAb level between two groups （P〉0.05）. Histopathological examination showed diffuse thyroid enlargement, great increase of ollicular epithelial cells and no obvious lymphocyte infiltration in 4 mice in experimental 1 group and 7 mice in experimental 2 group. The rate of Graves＇ disease modeling was significantly higher in experimental 2 group （70 %） than that in experimental 1 group （40%） （P〈0.05）. TSAb was positively correlated with FT4 in experimental 2 group （r= 0. 863, P=0. 006）. Conclusion It is feasible to establish Graves＇ disease mice models by inducing C57BL6 with

关 键 词：GRAVES病 猪甲状腺球蛋白 免疫诱导 小鼠 

分 类 号：R581[医药卫生—内分泌]