非水相脂肪酶催化阿魏酸淀粉酯的合成  

Lipase-catalyzed Synthesis of Starch Ferulate in Non-aqueous System

作　　者：李泉荟 辛嘉英[1,2] 王艳[1] 林美君[1] 夏春谷[2] 

机构地区：[1]哈尔滨商业大学黑龙江省高校食品科学与工程重点实验室,黑龙江哈尔滨150076 [2]中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室,甘肃兰州730000

出　　处：《精细化工》2016年第11期1266-1271,1294,共7页Fine Chemicals

基　　金：黑龙江省应用技术研究与开发项目(GC13C111);哈尔滨商业大学研究生创新科研基金项目(YJSCX2015-391HSD)~~

摘　　要：以玉米淀粉和阿魏酸乙酯为原料,利用脂肪酶催化合成了阿魏酸淀粉酯,对反应介质和脂肪酶进行了筛选,同时对影响合成阿魏酸淀粉酯反应的因素进行了探究,主要考察了底物摩尔比、酶添加量、反应时间及反应温度对反应的影响。采用紫外分光光度计对取代度进行测定,并以取代度为考察指标,确定了最佳反应条件:Novozym435脂肪酶为催化剂、异辛烷为反应介质、底物摩尔比n(阿魏酸乙酯)∶n(活化淀粉)=3∶1、酶添加量为10%(以活化淀粉质量计)、反应时间18 h、反应温度65℃,在该条件下,产物的最大取代度可达0.031,并通过FTIR以及1HNMR对产物结构进行了表征。Starch ferulate was synthesized via lipase-catalyzed transesterification of corn starch with ferulic acid ethyl ester in non-aqueous system. Different solvents and lipase employed were optimized.Meanwhile,the effects of reaction factors like mole ratio of substrate,addition of lipase,reaction temperature and time were investigated. The degree of substitution of the product was determined by ultraviolet spectrophotometer. The experimental results demonstrated that Novozym435 lipase and isooctane were the best catalyst and solvent,respectively. When molar ratio of ferulic acid ethyl ester to starch was 3 ∶ 1 and addition of lipase was 10%（ based on mass of starch）,starch ferulate with a maximum degree of substitution of 0. 031 was obtained,and its chemical structure was confirmed by FTIR and1 HNMR spectra.

关 键 词：阿魏酸乙酯 阿魏酸淀粉酯 Novozym435脂肪酶 转酯 淀粉化学品 

分 类 号：O621.3[理学—有机化学]